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<p>The primary sensor for environmental cues, cuticle is an essential agronomical trait. The extracellular cuticle proteome in crop plants is not well understood. A quantitative shotgun proteomics analysis on four economically relevant Brassicaceae members, <i>B. juncea, B. nigra, B. rapa,</i> and <i>R. sativus</i>, was performed. SignalP and SecretomeP predicted 323 secretory proteins, of which, 181 proteins were classically secreted and 140 proteins were non-classically secreted. QuickGO analysis revealed that most of these proteins had metabolic activity related to proteins (14%), lipids (14%), catalytic activity (19%), and defence response (18%). Some of the most abundant secreted proteins linked with lipid metabolism (<i>GDSL esterase lipase, ESM1</i>), catalytic activity (<i>glucanase</i>, <i>myrosinase</i>), and oxidoreductases (<i>peroxidase</i>, <i>ascorbate peroxidase</i>) were validated by activity assays and western blotting. Also, the abundant non-classical secreted proteins including <i>malate dehydrogenase</i>, <i>catalase</i>, and <i>fructose 1–6 bisphosphate aldolase</i> showed activities. According to the results of the current proteomics study, the active enzymes in the cuticle may serve a variety of functions at the first line of defence. DATA are available via ProteomeXchange with identifier-PXD056855<Emphasis Type="BoldItalic">.</Emphasis> Cutinsomes (esterified nanostructures) which are thought to be involved in cuticle assembly through non-enzymatic mechanism were synthesized in vitro and validated by FTIR and UV–visible spectroscopy. In future, cutinsomes may be studied for exploring their potential to form Bioplastics. This would utilize underutilized Brassica agrowastes to reduce environmental pollution caused by plastics.</p> Graphical Abstract <p></p>

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Proteome Analysis of Brassica Cuticle Opens a Treasure Trove for Crop Improvement Targets and Bioplastic

  • 期刊论文

  • 发表日期：

    2025年7月18日

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  • Meenakshi Arya,
  • Renu Deswal

摘要

The primary sensor for environmental cues, cuticle is an essential agronomical trait. The extracellular cuticle proteome in crop plants is not well understood. A quantitative shotgun proteomics analysis on four economically relevant Brassicaceae members, B. juncea, B. nigra, B. rapa, and R. sativus, was performed. SignalP and SecretomeP predicted 323 secretory proteins, of which, 181 proteins were classically secreted and 140 proteins were non-classically secreted. QuickGO analysis revealed that most of these proteins had metabolic activity related to proteins (14%), lipids (14%), catalytic activity (19%), and defence response (18%). Some of the most abundant secreted proteins linked with lipid metabolism (GDSL esterase lipase, ESM1), catalytic activity (glucanase, myrosinase), and oxidoreductases (peroxidase, ascorbate peroxidase) were validated by activity assays and western blotting. Also, the abundant non-classical secreted proteins including malate dehydrogenase, catalase, and fructose 1–6 bisphosphate aldolase showed activities. According to the results of the current proteomics study, the active enzymes in the cuticle may serve a variety of functions at the first line of defence. DATA are available via ProteomeXchange with identifier-PXD056855. Cutinsomes (esterified nanostructures) which are thought to be involved in cuticle assembly through non-enzymatic mechanism were synthesized in vitro and validated by FTIR and UV–visible spectroscopy. In future, cutinsomes may be studied for exploring their potential to form Bioplastics. This would utilize underutilized Brassica agrowastes to reduce environmental pollution caused by plastics.

Graphical Abstract