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<p>Oxidative stress is hypothesized as a hallmark for flower senescence in ethylene-insensitive species. Gamma-amino butyric acid (GABA) has recently emerged as a novel protective agent, known for its ability to respond and mitigate environmental stresses such as senescence.This study examines the effectiveness of GABA in alleviating postharvest oxidative stress and delaying senescence in tepals of <i>Lilium tigrinum</i>, an ethylene-insensitive flower. Harvested flower buds were treated with various concentrations of GABA 20&#xa0;mM, 40&#xa0;mM, 60&#xa0;mM, and 80&#xa0;mM. Flowers placed in distilled water (DW) served as control. Our findings revealed that 60&#xa0;mM mitigated senescence in <i>L. tigrinum</i>, enhancing the overall performance of the flowers. This non-proteinogenic amino acid elevated the antioxidant enzyme activities, prevented hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) accumulation, and maintained higher levels of total phenols, sugar fractions, soluble proteins, and proline content in tepal tissues of <i>L. tigrinum</i>. In addition, GABA improved membrane stability as compared to control which increased solution uptake. Furthermore, our results suggest that GABA delays senescence in <i>L. tigrinum</i> cut flowers primarily by downregulating the relative expression of <i>Lipoxygenase 1 (LOX1)</i> and <i>Senescence associated gene 12 (SAG12)</i> while upregulating the expression of the <i>Defender Against Death 1(DAD1)</i> gene in 60&#xa0;mM treated GABA in comparison to control. This upregulation/downregulation reinforces the antioxidant defense system by mitigating oxidative stress thereby enhancing the longevity of <i>L. tigrinum</i>.</p>

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GABA-Mediated Defense Mechanisms Against Oxidative Stress: A Case Study in Lilium tigrinum Cut Flowers

  • 期刊论文

  • 发表日期：

    2025年5月23日

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  • Moonisah Aftab,
  • Haris Yousuf Lone,
  • Aijaz A. Wani,
  • Mohmad Arief Zargar,
  • Inayatullah Tahir

摘要

Oxidative stress is hypothesized as a hallmark for flower senescence in ethylene-insensitive species. Gamma-amino butyric acid (GABA) has recently emerged as a novel protective agent, known for its ability to respond and mitigate environmental stresses such as senescence.This study examines the effectiveness of GABA in alleviating postharvest oxidative stress and delaying senescence in tepals of Lilium tigrinum, an ethylene-insensitive flower. Harvested flower buds were treated with various concentrations of GABA 20 mM, 40 mM, 60 mM, and 80 mM. Flowers placed in distilled water (DW) served as control. Our findings revealed that 60 mM mitigated senescence in L. tigrinum, enhancing the overall performance of the flowers. This non-proteinogenic amino acid elevated the antioxidant enzyme activities, prevented hydrogen peroxide (H2O2) accumulation, and maintained higher levels of total phenols, sugar fractions, soluble proteins, and proline content in tepal tissues of L. tigrinum. In addition, GABA improved membrane stability as compared to control which increased solution uptake. Furthermore, our results suggest that GABA delays senescence in L. tigrinum cut flowers primarily by downregulating the relative expression of Lipoxygenase 1 (LOX1) and Senescence associated gene 12 (SAG12) while upregulating the expression of the Defender Against Death 1(DAD1) gene in 60 mM treated GABA in comparison to control. This upregulation/downregulation reinforces the antioxidant defense system by mitigating oxidative stress thereby enhancing the longevity of L. tigrinum.