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<p>Avocado is one of the most important staple fruit crops worldwide, but various factors complicate its mass propagation. Somatic embryogenesis (SE) could address this issue; however, despite four decades of research, several challenges have hindered its implementation for this species. In this study, we conducted a de novo transcriptomic analysis to understand the molecular mechanisms behind the poor embryo maturation observed during avocado SE progression. We obtained a collection of 80,013 unigenes, with 7950 (9.93%) identified as differentially expressed across the four developmental stages we examined: cell suspension, early globular, late globular, and white-opaque embryos. Differential analysis of the transcripts evidenced a transcriptional alteration of many genes, particularly those associated with the biosynthesis of auxins and cytokinins, compared to plant species where SE occurs successfully. These findings were further supported by targeted metabolomic analysis. Overall, our results provide evidence of the dysregulated molecular features in avocados during SE and provide valuable resources for further investigation into somatic embryogenesis in this species.</p>

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Dysregulation in the Auxin and Cytokinin Signaling Pathways Intervenes in the Abnormal Development of Avocado (Persea americana Mill.) Somatic Embryos

  • 期刊论文

  • 发表日期：

    2025年3月20日

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  • Carol Alexis Olivares-García,
  • Jesús Alejandro Zamora-Briseño,
  • José Luis Lorenzo-Manzanarez,
  • Carolina Peña-Montes,
  • Víctor Manuel Loyola-Vargas,
  • Enrique Ibarra-Laclette,
  • Eliel Ruíz-May,
  • Martín Mata-Rosas

摘要

Avocado is one of the most important staple fruit crops worldwide, but various factors complicate its mass propagation. Somatic embryogenesis (SE) could address this issue; however, despite four decades of research, several challenges have hindered its implementation for this species. In this study, we conducted a de novo transcriptomic analysis to understand the molecular mechanisms behind the poor embryo maturation observed during avocado SE progression. We obtained a collection of 80,013 unigenes, with 7950 (9.93%) identified as differentially expressed across the four developmental stages we examined: cell suspension, early globular, late globular, and white-opaque embryos. Differential analysis of the transcripts evidenced a transcriptional alteration of many genes, particularly those associated with the biosynthesis of auxins and cytokinins, compared to plant species where SE occurs successfully. These findings were further supported by targeted metabolomic analysis. Overall, our results provide evidence of the dysregulated molecular features in avocados during SE and provide valuable resources for further investigation into somatic embryogenesis in this species.