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<p>Plant lesion mimic mutants (LMMs) are valuable for studying hypersensitive responses and disease resistance. In this study, we characterized a novel light-dependent LMM, <i>lmm6.1</i>, in rice. The <i>lmm6.1</i> mutant initially exhibited red rust-like spots on its leaves at the three- and four-leaf stages, which later progressed into large, dark rust-like lesions during rice development. Microscopic observations, histochemical characterizations, and physiological analyses revealed that the mutant displayed a lesion phenotype characterized by degenerated chloroplast structure, programmed cell death (PCD), excessive H<sub>2</sub>O<sub>2</sub> accumulation, increased malondialdehyde content, enhanced antioxidant enzyme activities, and decreased photosynthetic efficiency. Furthermore, genetic analysis indicated that <i>lmm6.1</i> was controlled by a recessive gene, which was finely mapped to a 187&#xa0;kb interval on chromosome 6. By integrating gene expression profiles and protein–protein interaction (PPI) networks, we identified six candidate genes: <i>ARFB1A</i>, <i>OSK10</i>, <i>CPN60BETA1</i>, <i>FSD2</i>, <i>ACBP2</i>, and <i>BCC1</i>. Notably, <i>CPN60BETA1</i>, <i>FSD2</i>, <i>ACBP2</i>, and <i>BCC1</i> were associated with resistance to rice blast and bacterial blight. Our study provides valuable insights for the future cloning and functional analysis of <i>lmm6.1</i>, offering new clues to understanding the mechanisms of plant disease resistance.</p>

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Characterization and Genetic Dissection of a Light-Dependent Lesion Mimic Mutant lmm6.1 in Rice

  • 期刊论文

  • 发表日期：

    2025年3月8日

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  • Yuan Niu,
  • Lingjin Yu,
  • Jiang Wu,
  • Guoliang Zhang

摘要

Plant lesion mimic mutants (LMMs) are valuable for studying hypersensitive responses and disease resistance. In this study, we characterized a novel light-dependent LMM, lmm6.1, in rice. The lmm6.1 mutant initially exhibited red rust-like spots on its leaves at the three- and four-leaf stages, which later progressed into large, dark rust-like lesions during rice development. Microscopic observations, histochemical characterizations, and physiological analyses revealed that the mutant displayed a lesion phenotype characterized by degenerated chloroplast structure, programmed cell death (PCD), excessive H2O2 accumulation, increased malondialdehyde content, enhanced antioxidant enzyme activities, and decreased photosynthetic efficiency. Furthermore, genetic analysis indicated that lmm6.1 was controlled by a recessive gene, which was finely mapped to a 187 kb interval on chromosome 6. By integrating gene expression profiles and protein–protein interaction (PPI) networks, we identified six candidate genes: ARFB1A, OSK10, CPN60BETA1, FSD2, ACBP2, and BCC1. Notably, CPN60BETA1, FSD2, ACBP2, and BCC1 were associated with resistance to rice blast and bacterial blight. Our study provides valuable insights for the future cloning and functional analysis of lmm6.1, offering new clues to understanding the mechanisms of plant disease resistance.