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<p>Fusarium Crown Rot (FCR), primarily caused by <i>Fusarium pseudograminearum</i>, is a prevalent disease that leads to significant yield loss at various growth stages of wheat. This study investigated the role of the beneficial endophytic fungus <i>Piriformospora indica</i> in enhancing disease resistance to FCR in wheat. The findings demonstrated that colonization by <i>P. indica</i> significantly promoted growth and development of wheat, increased root relative water content and intracellular antioxidant enzyme activity, and reduced the elevation in lipid peroxidation product content caused by <i>F. pseudograminearum</i> infection, particularly the content of malondialdehyde. Wheat precolonized with <i>P. indica</i> spores significantly reduced the disease index caused by <i>F. pseudograminearum</i> infection leading to a substantial increase in wheat’s resistance against FCR. Transcriptome sequencing revealed that colonization of <i>P. indica</i> reprogrammed the expression pattern of WRKY transcription factors involved in the MAPK pathway, with the majority being up-regulated; RT-qPCR confirmed these transcriptome sequencing results. Bioinformatics analysis revealed that TaWRKY gene family contains one conserved domains and is widely distributed on chromosomes 1, 2, 3, 5, and 7 of wheat. The high copy number of this gene family on chromosomes further emphasizes its crucial role in plant growth and development processes. Furthermore, metabolomics analysis demonstrated that the substantial increase in the content of gibberellin and auxin in wheat after <i>P. indica</i> colonization might be associated with the overall upregulation of the transcription factor WRKY. This study provides experimental and theoretical basis for further understanding wheat’s resistance to FCR mediated by <i>P. indica</i> colonization.</p>

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WRKY Transcription Factors are Required in Piriformospora indica-Induced Resistance to Fusarium Crown Rot in Wheat

  • 期刊论文

  • 发表日期：

    2025年2月19日

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  • Liang Li,
  • Fuyan Qi,
  • Ruiying Hao,
  • Zhenghui Bi,
  • Xiurong Yang

摘要

Fusarium Crown Rot (FCR), primarily caused by Fusarium pseudograminearum, is a prevalent disease that leads to significant yield loss at various growth stages of wheat. This study investigated the role of the beneficial endophytic fungus Piriformospora indica in enhancing disease resistance to FCR in wheat. The findings demonstrated that colonization by P. indica significantly promoted growth and development of wheat, increased root relative water content and intracellular antioxidant enzyme activity, and reduced the elevation in lipid peroxidation product content caused by F. pseudograminearum infection, particularly the content of malondialdehyde. Wheat precolonized with P. indica spores significantly reduced the disease index caused by F. pseudograminearum infection leading to a substantial increase in wheat’s resistance against FCR. Transcriptome sequencing revealed that colonization of P. indica reprogrammed the expression pattern of WRKY transcription factors involved in the MAPK pathway, with the majority being up-regulated; RT-qPCR confirmed these transcriptome sequencing results. Bioinformatics analysis revealed that TaWRKY gene family contains one conserved domains and is widely distributed on chromosomes 1, 2, 3, 5, and 7 of wheat. The high copy number of this gene family on chromosomes further emphasizes its crucial role in plant growth and development processes. Furthermore, metabolomics analysis demonstrated that the substantial increase in the content of gibberellin and auxin in wheat after P. indica colonization might be associated with the overall upregulation of the transcription factor WRKY. This study provides experimental and theoretical basis for further understanding wheat’s resistance to FCR mediated by P. indica colonization.