Rapeseed (Brassica napus) is one of the most essential oil seeds with spring and autumn cultivars. One of the most significant abiotic stresses it faces is salinity stress, which reduces the osmotic potential and nutrient absorption by the plant. One solution to combat abiotic stresses is the use of plant growth-promoting bacteria (PGPB). Acyl Homoserine Lactones (AHLs) molecules play a role in the quorum sensing (QS) system, which helps plants develop resistance to stress. Genes dependent on ABA in the salt signaling pathway, such as: SOS1, SOS2, SOS3, P5CS1, ADH, RD22 and ERD1 genes, were studied along with genes independent of the ABA pathway. The highest positive correlation in the root tissue and S200B+ treatment of rapeseed Hyola #4815 was found between the SOS3 gene and the ERD1 gene + 0.952, while the lowest correlation was between the SOS1 gene and the RD22 gene − 0.968 showed. The RD22 gene showed the highest negative correlation with the ADH3 gene − 0.968 in root tissue. The leaf tissue, the highest positive correlation was observed between the SOS1 and ADH3 genes + 0.742, while the RD22 gene had the lowest correlation of − 0.993 with the ADH3 gene. The physiological characteristics of rapeseed Hayola #4815 were studied after the application of Aeromonas hydrophila.There was significantly an increase in PFW (13 g), DW (3.66 g), SL (45.6 cm), SFW (7.33 g), RL (12 cm) and RFW (3.66 g) compared to the control at p < 0.05. The sodium content in the roots (1.53 mg/g DW−1) and leaves (2.87 mg/g DW−1) of S200B+ significantly increased compared to the control (COB−). Leaf potassium concentration (18.70 mg/g DW−1) increased in seedlings subjected to salt stress without bacteria (S200B−) compare to the control (COB−), while root potassium concentration (17.98 mg/g DW−1) increased due to the application of COB+. Proline levels in leaf and root tissue decreased in the treatments compared to the control. Chlorophyll a (1.02 mg/gFW−1), b (0.36 mg/gFW−1), and total content (0.36 mg/gFW−1) decreased in S200B+ compare to the control (2.29 mg/gFW−1, COB−), while carotenoid content (0.55 mg/gFW−1)increased. In conclusion, this research provides new insights to improve the sustainability of rapeseed fields. In additions; comparison between treatments (+B versus B−) suggested their contribution toward salt mitigation and plant growth improvement. However, our findings were conducted in a greenhouse under controlled conditions, and further studies are needed in field conditions to validate these effects only after implementation of biosafety parameters because Aeromonas spp have clinical significant. This study could be helpful solution better cropping in the salt affected soil on a large scale.

Aeromonas hydrophila by Quorum Sensing Auto-Inducers on Growth Amelioration, Enhance Salt Tolerance and Mechanism of Encoding Genes in Rapeseed

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发表日期：

2025年2月7日

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Neda Hariri,
Karim Sorkheh,
Leila Nejadsadeghi

摘要

Rapeseed (Brassica napus) is one of the most essential oil seeds with spring and autumn cultivars. One of the most significant abiotic stresses it faces is salinity stress, which reduces the osmotic potential and nutrient absorption by the plant. One solution to combat abiotic stresses is the use of plant growth-promoting bacteria (PGPB). Acyl Homoserine Lactones (AHLs) molecules play a role in the quorum sensing (QS) system, which helps plants develop resistance to stress. Genes dependent on ABA in the salt signaling pathway, such as: SOS1, SOS2, SOS3, P5CS1, ADH, RD22 and ERD1 genes, were studied along with genes independent of the ABA pathway. The highest positive correlation in the root tissue and S200B+ treatment of rapeseed Hyola #4815 was found between the SOS3 gene and the ERD1 gene + 0.952, while the lowest correlation was between the SOS1 gene and the RD22 gene − 0.968 showed. The RD22 gene showed the highest negative correlation with the ADH3 gene − 0.968 in root tissue. The leaf tissue, the highest positive correlation was observed between the SOS1 and ADH3 genes + 0.742, while the RD22 gene had the lowest correlation of − 0.993 with the ADH3 gene. The physiological characteristics of rapeseed Hayola #4815 were studied after the application of Aeromonas hydrophila.There was significantly an increase in PFW (13 g), DW (3.66 g), SL (45.6 cm), SFW (7.33 g), RL (12 cm) and RFW (3.66 g) compared to the control at p < 0.05. The sodium content in the roots (1.53 mg/g DW⁻¹) and leaves (2.87 mg/g DW⁻¹) of S200B+ significantly increased compared to the control (COB−). Leaf potassium concentration (18.70 mg/g DW⁻¹) increased in seedlings subjected to salt stress without bacteria (S200B−) compare to the control (COB−), while root potassium concentration (17.98 mg/g DW⁻¹) increased due to the application of COB+. Proline levels in leaf and root tissue decreased in the treatments compared to the control. Chlorophyll a (1.02 mg/gFW⁻¹), b (0.36 mg/gFW⁻¹), and total content (0.36 mg/gFW⁻¹) decreased in S200B+ compare to the control (2.29 mg/gFW⁻¹, COB−), while carotenoid content (0.55 mg/gFW⁻¹)increased. In conclusion, this research provides new insights to improve the sustainability of rapeseed fields. In additions; comparison between treatments (+B versus B−) suggested their contribution toward salt mitigation and plant growth improvement. However, our findings were conducted in a greenhouse under controlled conditions, and further studies are needed in field conditions to validate these effects only after implementation of biosafety parameters because Aeromonas spp have clinical significant. This study could be helpful solution better cropping in the salt affected soil on a large scale.