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<p>The Chinese shrimp (<i>Fenneropenaeus chinensis</i>) is one of the most valuable cultured penaeid species. However, overfishing and inbreeding depression have caused marked declines in both wild stocks and aquaculture production. As the main edible portion, muscle tissue plays a decisive role in growth performance, one of the most critical economic traits in shrimp breeding. Myogenic differentiation factor D (MyoD) is a key positive regulator of myogenesis and skeletal muscle growth in vertebrates; however, knowledge of its role in crustacean muscle development remains limited. In this study, we identified and characterized the <i>MyoD</i> homolog from Chinese shrimp (<i>FcMyoD</i>) and investigated its potential function in muscle growth. The full-length <i>FcMyoD</i> cDNA is 1 484 bp in length and encodes a 210-amino-acid protein. Compared with <i>MyoD</i> from <i>Litopenaeus vannamei</i> (<i>LvMyoD</i>), <i>FcMyoD</i> harbors a frameshift mutation that truncates of the C-terminal region. In adult shrimp, <i>FcMyoD</i> expression was highest in muscle tissue, and muscle <i>FcMyoD</i> expression in large individuals was significantly higher than in small individuals. Following 36 d of continuous double-stranded RNA (dsRNA) targeting <i>FcMyoD</i>, the muscle growth rate in the dsRNA group was markedly lower than in the phosphate buffer saline (PBS) group. Histological analysis further revealed reduces muscle fiber diameter and narrower inter-fiber spaces in the dsRNA group. These results suggest that <i>FcMyoD</i> may act as a positive regulator of muscle formation and growth in Chinese shrimp. This study provides important information for the role of <i>MyoD</i> in crustacean myogenesis and muscle growth and a basic insight for future molecular breeding strategies to improve the growth traits in farmed shrimp.</p>

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Potential roles of MyoD in promoting myogenesis and muscle growth in Fenneropenaeus chinensis

  • 期刊论文

  • 发表日期：

    2026年7月25日

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  • Xia Lu,
  • Zebin Zhang,
  • Nan Jiang,
  • Yongzheng Tang,
  • Fangrui Lou,
  • Yan Liu,
  • Tingjin Lü,
  • Xuewei Qu

摘要

The Chinese shrimp (Fenneropenaeus chinensis) is one of the most valuable cultured penaeid species. However, overfishing and inbreeding depression have caused marked declines in both wild stocks and aquaculture production. As the main edible portion, muscle tissue plays a decisive role in growth performance, one of the most critical economic traits in shrimp breeding. Myogenic differentiation factor D (MyoD) is a key positive regulator of myogenesis and skeletal muscle growth in vertebrates; however, knowledge of its role in crustacean muscle development remains limited. In this study, we identified and characterized the MyoD homolog from Chinese shrimp (FcMyoD) and investigated its potential function in muscle growth. The full-length FcMyoD cDNA is 1 484 bp in length and encodes a 210-amino-acid protein. Compared with MyoD from Litopenaeus vannamei (LvMyoD), FcMyoD harbors a frameshift mutation that truncates of the C-terminal region. In adult shrimp, FcMyoD expression was highest in muscle tissue, and muscle FcMyoD expression in large individuals was significantly higher than in small individuals. Following 36 d of continuous double-stranded RNA (dsRNA) targeting FcMyoD, the muscle growth rate in the dsRNA group was markedly lower than in the phosphate buffer saline (PBS) group. Histological analysis further revealed reduces muscle fiber diameter and narrower inter-fiber spaces in the dsRNA group. These results suggest that FcMyoD may act as a positive regulator of muscle formation and growth in Chinese shrimp. This study provides important information for the role of MyoD in crustacean myogenesis and muscle growth and a basic insight for future molecular breeding strategies to improve the growth traits in farmed shrimp.