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<p>The Akoya pearl oyster <i>Pinctada fucata</i> is a key model for studying bivalve sex determination and reproduction, yet its reproductive molecular mechanisms remain unclear. The Tudor domain-containing proteins (TDRDs) are critical for animal reproductive development, making their roles in <i>P. fucata</i> worthy of exploration. In this study, we cloned three TDRDs family genes (<i>PfTDRD5</i>, <i>PfTDRD7-like</i>, <i>PfTDRD9-like</i>) from <i>P. fucata</i>, all containing the signature Tudor domain. Additionally, <i>PfTDRD5</i> has two Limkain, Oskar, and Tudor containing proteins 5 and 7 (LOTUS) domains and one La and related RNA-binding proteins (LARPs) superfamily domain. Phylogenetic analysis showed evolutionary conservation with homologous genes from other species. qPCR revealed differential expression: <i>PfTDRD5</i> and <i>PfTDRD7-like</i> were highly expressed in female tissues (highest in ovary) and peaked in female gonadal resting stage, while <i>PfTDRD9-like</i> was more abundant in male tissues and increased with gamete maturation. In-situ hybridization localized their expression to ovarian/testicular follicles and gametes at various stages. All three genes were highly expressed from 4 to 16 cell embryonic stages, suggesting roles in early embryonic development. RNA interference (RNAi) knockdown caused severe gonadal damage, oocyte degeneration, and reduced sperm quantity/motility. This study provided a foundation for exploring TDRDs’ roles in bivalve gonadal development, enhancing understanding of reproductive molecular mechanisms in <i>P. fucata</i> and related species.</p>

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Cloning, tissue expression, and functional analysis of PfTDRD5, PfTDRD7-like, and PfTDRD9-like genes in pearl oyster Pinctada fucata

  • 期刊论文

  • 发表日期：

    2026年3月7日

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  • Tangrongjun Guo,
  • Ying Guo,
  • Pei Wang,
  • Weiwei Chen,
  • Ruolin Zhu,
  • Lirong Bai,
  • Dahui Yu

摘要

The Akoya pearl oyster Pinctada fucata is a key model for studying bivalve sex determination and reproduction, yet its reproductive molecular mechanisms remain unclear. The Tudor domain-containing proteins (TDRDs) are critical for animal reproductive development, making their roles in P. fucata worthy of exploration. In this study, we cloned three TDRDs family genes (PfTDRD5, PfTDRD7-like, PfTDRD9-like) from P. fucata, all containing the signature Tudor domain. Additionally, PfTDRD5 has two Limkain, Oskar, and Tudor containing proteins 5 and 7 (LOTUS) domains and one La and related RNA-binding proteins (LARPs) superfamily domain. Phylogenetic analysis showed evolutionary conservation with homologous genes from other species. qPCR revealed differential expression: PfTDRD5 and PfTDRD7-like were highly expressed in female tissues (highest in ovary) and peaked in female gonadal resting stage, while PfTDRD9-like was more abundant in male tissues and increased with gamete maturation. In-situ hybridization localized their expression to ovarian/testicular follicles and gametes at various stages. All three genes were highly expressed from 4 to 16 cell embryonic stages, suggesting roles in early embryonic development. RNA interference (RNAi) knockdown caused severe gonadal damage, oocyte degeneration, and reduced sperm quantity/motility. This study provided a foundation for exploring TDRDs’ roles in bivalve gonadal development, enhancing understanding of reproductive molecular mechanisms in P. fucata and related species.