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<p>The effects and mechanism of NaAc addition and supply modes growth in both green and red stages of <i>Haematococcus pluvialis</i> were investigated, and 1-g/L NaAc was found the optimal initial concentration for the two stages. On this basis, the extra supplement of 1-g/L NaAc on Day 6 of the green stage or on Day 3 of the red stage could further increase the accumulation of biomass. Compared with autotrophic control, the optimal NaAc supply mode increased the dry weight by 135.71% (green stage) and 91.11% (red stage), while astaxanthin and lipid were increased by 56.9% and 42.86% in red stage, respectively. Gene expression detection revealed that acetyl-CoA synthetase (<i>acs</i>) <i>acs2, acs3</i>, and <i>acs5</i> were primarily responsible for the NaAc uptake during the green stage, and <i>acs4</i> played a critical role in stress response during the red stage, additionally, key genes associated with acetate metabolism, astaxanthin synthesis, and lipid synthesis, were significantly upregulated under optimal NaAc supply mode. The findings provide practical and theoretical insights for NaAc use in <i>Haematococcus pluvialis</i>.</p>

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Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of Haematococcus pluvialis

  • 期刊论文

  • 发表日期：

    2026年1月26日

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  • Yanbin Li,
  • Weizhao Han,
  • Na Li,
  • Tian Xie,
  • Xin Tan,
  • Song Tang,
  • Yequan Sheng,
  • Bangxiang He

摘要

The effects and mechanism of NaAc addition and supply modes growth in both green and red stages of Haematococcus pluvialis were investigated, and 1-g/L NaAc was found the optimal initial concentration for the two stages. On this basis, the extra supplement of 1-g/L NaAc on Day 6 of the green stage or on Day 3 of the red stage could further increase the accumulation of biomass. Compared with autotrophic control, the optimal NaAc supply mode increased the dry weight by 135.71% (green stage) and 91.11% (red stage), while astaxanthin and lipid were increased by 56.9% and 42.86% in red stage, respectively. Gene expression detection revealed that acetyl-CoA synthetase (acs) acs2, acs3, and acs5 were primarily responsible for the NaAc uptake during the green stage, and acs4 played a critical role in stress response during the red stage, additionally, key genes associated with acetate metabolism, astaxanthin synthesis, and lipid synthesis, were significantly upregulated under optimal NaAc supply mode. The findings provide practical and theoretical insights for NaAc use in Haematococcus pluvialis.