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<p>To enhance the application of environmental DNA (eDNA) technology in zoobenthos monitoring, we systematically evaluated the effectiveness of five environmental samples in detecting zoobenthos communities: water, filtered water, sediment, detritus, and bulk samples. A novel wet-funnel method was developed for efficiently collecting bulk samples, enabling the isolation of small, hygrophilous zoobenthos with minimal disturbance. This approach significantly enhanced DNA extraction efficiency while reducing noise in sequencing data. Results demonstrate that the species compositions of bulk samples processed with wet funnels were most comparable to traditional morphological identification methods. Additionally, combining bulk samples and sediment samples captured the richest species information across taxonomic groups, with a notable improvement in detecting annelids and mollusks. These findings highlight that wet funnels are a practical tool for streamlining eDNA workflows and optimizing zoobenthos monitoring. This study contributed to the development of standardized protocols for freshwater biodiversity assessments using eDNA metabarcoding.</p>

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Wet-funnel and sediment are essential supplements for eDNA sampling strategy in lakes

  • 期刊论文

  • 发表日期：

    2026年5月29日

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  • Jiefeng Yu,
  • Yajing He,
  • Kena Shi,
  • Yongjing Zhao,
  • Yongde Cui

摘要

To enhance the application of environmental DNA (eDNA) technology in zoobenthos monitoring, we systematically evaluated the effectiveness of five environmental samples in detecting zoobenthos communities: water, filtered water, sediment, detritus, and bulk samples. A novel wet-funnel method was developed for efficiently collecting bulk samples, enabling the isolation of small, hygrophilous zoobenthos with minimal disturbance. This approach significantly enhanced DNA extraction efficiency while reducing noise in sequencing data. Results demonstrate that the species compositions of bulk samples processed with wet funnels were most comparable to traditional morphological identification methods. Additionally, combining bulk samples and sediment samples captured the richest species information across taxonomic groups, with a notable improvement in detecting annelids and mollusks. These findings highlight that wet funnels are a practical tool for streamlining eDNA workflows and optimizing zoobenthos monitoring. This study contributed to the development of standardized protocols for freshwater biodiversity assessments using eDNA metabarcoding.