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<p>The mud crab (<i>Scylla paramamosain</i>) has been successfully cultivated in chloride-type low-salinity alkaline waters, yet the molecular mechanisms underlying its adaptation to this environment remain elusive. This study seeks to uncover the adaptation mechanisms of the antennal gland of mud crab under acute chloride-type low-salinity alkaline water stress. Three-hundred crabs were randomly selected and divided into two groups: the low salinity (LS, salinity of 2.2) group and the control group (CK, salinity of 23.4). Following a 120-h experimental period, the antennal glands were sampled for transcriptomic analysis. Experimental results show that acute chloride-type low-salinity saline-alkaline water stress led to a significant decline in the survivorship of mud crab. Through comparative transcriptomic analysis, we identified 923 differentially expressed genes (DEGs), among which 272 were upregulated and 651 were downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of these DEGs are closely linked to functions such as energy metabolism and osmoregulation. Notably, the pathways involving oxidoreductase activity, oxidative phosphorylation, symporter activity, and intracellular calcium-activated chloride channels were significantly enriched, highlighting their pivotal roles in enhancing the osmoregulatory capacity of mud crab and maintaining internal homeostasis in chloride-type low-salinity alkaline environments. Additionally, the upregulation of genes such as <i>SLC6A9</i>, <i>SLC6A4</i>, and <i>FH</i> further facilitated transmembrane ion transport and energy metabolism, thereby reinforcing intracellular isosmotic regulation. However, these controls also pose a potential risk of oxidative stress. Therefore, the antennal gland plays a crucial role in the response of mud crab to acute chloride-type low-salinity alkaline water stress. This study provides new insights into the adaptive mechanisms of mud crab and lays a theoretical foundation for enhancing chloride-type low-salinity alkaline water aquaculture techniques.</p>

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Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Jinwei Wang,
  • Xiaosong Jiang,
  • Changkao Mu,
  • Chunlin Wang,
  • Huan Wang

摘要

The mud crab (Scylla paramamosain) has been successfully cultivated in chloride-type low-salinity alkaline waters, yet the molecular mechanisms underlying its adaptation to this environment remain elusive. This study seeks to uncover the adaptation mechanisms of the antennal gland of mud crab under acute chloride-type low-salinity alkaline water stress. Three-hundred crabs were randomly selected and divided into two groups: the low salinity (LS, salinity of 2.2) group and the control group (CK, salinity of 23.4). Following a 120-h experimental period, the antennal glands were sampled for transcriptomic analysis. Experimental results show that acute chloride-type low-salinity saline-alkaline water stress led to a significant decline in the survivorship of mud crab. Through comparative transcriptomic analysis, we identified 923 differentially expressed genes (DEGs), among which 272 were upregulated and 651 were downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of these DEGs are closely linked to functions such as energy metabolism and osmoregulation. Notably, the pathways involving oxidoreductase activity, oxidative phosphorylation, symporter activity, and intracellular calcium-activated chloride channels were significantly enriched, highlighting their pivotal roles in enhancing the osmoregulatory capacity of mud crab and maintaining internal homeostasis in chloride-type low-salinity alkaline environments. Additionally, the upregulation of genes such as SLC6A9, SLC6A4, and FH further facilitated transmembrane ion transport and energy metabolism, thereby reinforcing intracellular isosmotic regulation. However, these controls also pose a potential risk of oxidative stress. Therefore, the antennal gland plays a crucial role in the response of mud crab to acute chloride-type low-salinity alkaline water stress. This study provides new insights into the adaptive mechanisms of mud crab and lays a theoretical foundation for enhancing chloride-type low-salinity alkaline water aquaculture techniques.