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<p>Intraspecific conflict induced by the innate aggressiveness is one of the main reasons for the extremely low survival rate in mud crab <i>Scylla paramamosain</i> aquaculture, which have impeded the sustainable culture of the species. In this study, we first classified and quantified the aggressive behavior, and established a crab aggressive behavior model, laying the foundation for subsequent research on evaluating combat intensity. The contents of 5-hydroxytryptamine (5-HT), dopamine (DA), and cAMP in the hemolymph of the mud crabs before and after fighting were measured by high-performance liquid chromatography-mass spectrometry (HPLC-MS), and the mud crabs exhibited a significant increase of 5-HT (<i>P</i>&lt;0.05), while the DA and cAMP decreased significantly (<i>P</i>&lt;0.05). In addition, we applied EthoVision to examine the changes of the crab behavior after DA administration. After 0.5 h of injection, the movement speed, distance, duration of aggressive behavior, and intensity of aggression in the high concentration DA group were significantly higher than those in the saline injection group and the untreated control group (<i>P</i>&lt;0.05). The results of real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that the expression of DA1 in the thoracic ganglia of the mud crabs was significantly down-regulated in the DA injection group, and the aggressive behavior was weakened. Conversely, DA1 expression was up-regulated when aggressive behavior was strengthened. Besides, there were significant differences in the expression levels of receptor expression genes including 5-HT1, 5-HT2, and crustacean hyperglycemic hormone (CHH) in different tissues, indicating that the alteration of aggressive behavior of the mud crab after injection with different concentrations of DA could be regulated by changes in the expression levels of corresponding receptor genes. Our results contribute to a deeper analysis of the aggressive behavior mechanism of the mud crabs and provide a theoretical basis for reducing fighting-related mortalities in aquaculture.</p>

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Dopamine mediates the link between aggressive behavior and biogenic amine receptor genes in mud crab Scylla paramamosain

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Yuanyuan Fu,
  • Xinlian Huang,
  • Lei Liu,
  • Wei Zhai,
  • Sixiang Wang,
  • Congying He

摘要

Intraspecific conflict induced by the innate aggressiveness is one of the main reasons for the extremely low survival rate in mud crab Scylla paramamosain aquaculture, which have impeded the sustainable culture of the species. In this study, we first classified and quantified the aggressive behavior, and established a crab aggressive behavior model, laying the foundation for subsequent research on evaluating combat intensity. The contents of 5-hydroxytryptamine (5-HT), dopamine (DA), and cAMP in the hemolymph of the mud crabs before and after fighting were measured by high-performance liquid chromatography-mass spectrometry (HPLC-MS), and the mud crabs exhibited a significant increase of 5-HT (P<0.05), while the DA and cAMP decreased significantly (P<0.05). In addition, we applied EthoVision to examine the changes of the crab behavior after DA administration. After 0.5 h of injection, the movement speed, distance, duration of aggressive behavior, and intensity of aggression in the high concentration DA group were significantly higher than those in the saline injection group and the untreated control group (P<0.05). The results of real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that the expression of DA1 in the thoracic ganglia of the mud crabs was significantly down-regulated in the DA injection group, and the aggressive behavior was weakened. Conversely, DA1 expression was up-regulated when aggressive behavior was strengthened. Besides, there were significant differences in the expression levels of receptor expression genes including 5-HT1, 5-HT2, and crustacean hyperglycemic hormone (CHH) in different tissues, indicating that the alteration of aggressive behavior of the mud crab after injection with different concentrations of DA could be regulated by changes in the expression levels of corresponding receptor genes. Our results contribute to a deeper analysis of the aggressive behavior mechanism of the mud crabs and provide a theoretical basis for reducing fighting-related mortalities in aquaculture.