Identification and construction of lncRNA-miRNA-mRNA ceRNA networks associated with temperature changes in Sebastiscus marmoratus

Sebastiscus marmoratus is one of the ideal fish species for offshore breeding, and temperature changes directly affect its physiological process. We performed whole-transcriptome sequencing of the liver tissues of S. marmoratus under heat stress (25 °C), normal condition (20 °C, the control), and cold stress (15 °C). A total of 376 differentially expressed genes (DEGs), 147 differentially expressed lncRNAs (DELs), and 40 differentially expressed miRNAs (DEMis) were detected under heat stress; 59 DEGs, 59 DELs, and 44 DEMis were detected under cold stress. Furthermore, a competing endogenous RNA regulatory network for the functional interaction of lncRNA-miRNA-mRNA was constructed, and GO and KEGG enrichment analyses showed that genes involved in maintaining homeostasis or adjusting to stress and stimulation were strongly activated during heat stress. Including heat-shock protein-related genes Hsp70, FKBP4, and Hspa4a regulated by dre-mir-205-5p; energy metabolism-related genes GCK, g6pca, and RFK regulated by dre-miR-205-5p, dre-miR-145-5p, novel_441, TCONS_00023692, and tcon_00095578; and immune-related genes SCAF, NLRC3, per1b, herc4, MafG, and KLHL29 regulated by dre-miR-456, novel_640, novel_163, TCONS_00079377, TCONS_00063590, and TCONS_000605708. Our findings provide new insights into the adaptation of S. marmoratus to acute temperature changes.