Phytopathogenic fungus-assisted synthesis of polycrystalline TiO₂ nanorods: Characterization, growth mechanism, and antibacterial activity
摘要
Biosynthesis enables the eco-friendly production of diverse nanostructures without harmful chemicals. In this work, a biosynthetic method has been successfully used to synthesize titanium oxide nanorods by employing the phytopathogenic fungus Fusarium solani. The plausible growth mechanism for the formation of biogenic TiO2 nanorods is presented. Thermogravimetric Analysis (TGA) and Differential Thermal Analysis (DTA) suggested the crystallization temperature at 450℃. X-ray Diffraction (XRD) pattern substantiated the presence of polycrystalline anatase phase TiO2. Fourier Transform Infrared Spectroscopy was used to examine how different functional groups in the Fusarium solani fungus extraction interacted to produce TiO2 nanoparticles. Energy Dispersive X-ray Spectroscopy (EDS) in conjunction with Field Emission Scanning Electron Microscopy (FESEM) verified the production of TiO2 nanorods with a diameter range between 11 and 15 nm. Raman spectroscopy revealed active vibrational modes consistent with the anatase phase of TiO2. The growth mechanism involves the interaction between Ti4+ ions and biomolecules from the fungal supernatant, leading to the stabilization and formation of TiO2 nanorods, which is discussed in detail. The disc diffusion method was used to evaluate the antibacterial properties of biogenic TiO2 nanorods against the bacterial strains, namely Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumonia, and the observed ZOI values are 16 mm, 12 mm, 20 mm, and 14 mm, respectively. Escherichia coli showed greater susceptibility to the synthesized material than the other bacterial strains.