<p>Bacterial resistance to antibiotics is a significant concern. Developing novel antibiotics frequently fails to address the swiftly escalating bacterial resistance and necessitates inventive strategies to tackle bacterial illnesses. The size-dependent antibacterial efficacy of Ni<sub>1− <i>x</i></sub>Cu<sub><i>x</i></sub>Fe<sub>2</sub>O<sub>4</sub> nanocomposites was examined utilizing Ni<sub>1− <i>x</i></sub>Cu<sub><i>x</i></sub>Fe<sub>2</sub>O<sub>4</sub> compositions with substitution values <i>x</i> = 0.2, 0.4, and 0.6, synthesized by the sol-gel auto-combustion technique at a consistent calcination temperature of 425&#xa0;°C for 3&#xa0;h. The X-ray diffraction pattern has been employed to validate the single-phase cubic spinel structure. The average size of the crystallite increases from 37.072&#xa0;nm to 42.288&#xa0;nm as the substitution process varies from <i>x</i> = 0.2 to <i>x</i> = 0.6, resulting in crystalline size growth. The microstructural investigation was performed using the FESEM technique, revealing that the average particle size grows from 39.429&#xa0;nm to 45.610&#xa0;nm with heightened copper substitution. The TEM images reveal a nanoparticle structure characterized by some agglomeration, the particles exhibiting a spherical morphology, and their sizes correspond well with those obtained from XRD analyses. The FTIR examination corroborated the spinel structure of the ferrite system. Two prominent absorption bands, <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="339_2025_8893_Article_IEq1.gif" Format="GIF" Height="19" Rendition="HTML" Resolution="72" Type="Linedraw" Width="28" /> </InlineMediaObject> <EquationSource Format="TEX">\(\:\left({v}_{2}\right)\)</EquationSource> </InlineEquation> corresponding to the octahedral site and <InlineEquation ID="IEq2"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="339_2025_8893_Article_IEq2.gif" Format="GIF" Height="19" Rendition="HTML" Resolution="72" Type="Linedraw" Width="28" /> </InlineMediaObject> <EquationSource Format="TEX">\(\:\left({v}_{1}\right)\)</EquationSource> </InlineEquation> to the tetrahedral site, are evident in the spectra of the FTIR. These bands are located in approximately 560.22–567.93&#xa0;cm<sup>−1</sup> and 395.34-402.09&#xa0;cm<sup>−1</sup>, respectively. The magnetization investigations indicate that the Ni<sub>1− <i>x</i></sub>Cu<sub><i>x</i></sub>Fe<sub>2</sub>O<sub>4</sub> nanocomposites have soft ferromagnetic characteristics, with a decrease in saturation magnetization from 49.2964 to 32.8189 emu/g and a reduction in coercivity from 110.6406 to 41.9433 Oe as the Cu<sup>2+</sup> substitution increases. Surface analysis has been conducted using the synthesized nanocomposites’ isothermal BET methodology. The Ni<sub>1− <i>x</i></sub>Cu<sub><i>x</i></sub>Fe<sub>2</sub>O<sub>4</sub> nanocomposites exhibited zeta potentials of -46.6, -54.5, and − 52.5 mV, respectively. Enhanced antibacterial activity was demonstrated in Ni<sub>1− <i>x</i></sub>Cu<sub><i>x</i></sub>Fe<sub>2</sub>O<sub>4</sub> nanocomposites with decreased copper substitution. The improvement in antibacterial activity was attributed to crystallinity and shape. When the compensation ratio for copper was <i>x</i> = 0.2, antibacterial testing using the agar well diffusion technique revealed that <i>Klebsiella pneumoniae</i> showed the highest effectiveness; <i>Neisseria gonorrhoeae</i> followed, then <i>Bacillus subtilis</i>, and <i>Enterococcus faecalis</i>. These intriguing findings highlight the unique inhibitory properties of biocompatible nickel-copper ferrites in augmenting bacterial eradication, as well as their promising potential as effective antimicrobial agents with possible applications in antimicrobial coatings and biomedical devices.</p>

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Structural, magnetic, and antibacterial properties of Ni1− xCuxFe2O4 nanocomposites: influence of Cu substitution

  • Tahseen H. Mubarak,
  • Mohammed B. Jumaa,
  • Ali M. Mohammad

摘要

Bacterial resistance to antibiotics is a significant concern. Developing novel antibiotics frequently fails to address the swiftly escalating bacterial resistance and necessitates inventive strategies to tackle bacterial illnesses. The size-dependent antibacterial efficacy of Ni1− xCuxFe2O4 nanocomposites was examined utilizing Ni1− xCuxFe2O4 compositions with substitution values x = 0.2, 0.4, and 0.6, synthesized by the sol-gel auto-combustion technique at a consistent calcination temperature of 425 °C for 3 h. The X-ray diffraction pattern has been employed to validate the single-phase cubic spinel structure. The average size of the crystallite increases from 37.072 nm to 42.288 nm as the substitution process varies from x = 0.2 to x = 0.6, resulting in crystalline size growth. The microstructural investigation was performed using the FESEM technique, revealing that the average particle size grows from 39.429 nm to 45.610 nm with heightened copper substitution. The TEM images reveal a nanoparticle structure characterized by some agglomeration, the particles exhibiting a spherical morphology, and their sizes correspond well with those obtained from XRD analyses. The FTIR examination corroborated the spinel structure of the ferrite system. Two prominent absorption bands, \(\:\left({v}_{2}\right)\) corresponding to the octahedral site and \(\:\left({v}_{1}\right)\) to the tetrahedral site, are evident in the spectra of the FTIR. These bands are located in approximately 560.22–567.93 cm−1 and 395.34-402.09 cm−1, respectively. The magnetization investigations indicate that the Ni1− xCuxFe2O4 nanocomposites have soft ferromagnetic characteristics, with a decrease in saturation magnetization from 49.2964 to 32.8189 emu/g and a reduction in coercivity from 110.6406 to 41.9433 Oe as the Cu2+ substitution increases. Surface analysis has been conducted using the synthesized nanocomposites’ isothermal BET methodology. The Ni1− xCuxFe2O4 nanocomposites exhibited zeta potentials of -46.6, -54.5, and − 52.5 mV, respectively. Enhanced antibacterial activity was demonstrated in Ni1− xCuxFe2O4 nanocomposites with decreased copper substitution. The improvement in antibacterial activity was attributed to crystallinity and shape. When the compensation ratio for copper was x = 0.2, antibacterial testing using the agar well diffusion technique revealed that Klebsiella pneumoniae showed the highest effectiveness; Neisseria gonorrhoeae followed, then Bacillus subtilis, and Enterococcus faecalis. These intriguing findings highlight the unique inhibitory properties of biocompatible nickel-copper ferrites in augmenting bacterial eradication, as well as their promising potential as effective antimicrobial agents with possible applications in antimicrobial coatings and biomedical devices.