Citric acid modified Fe-BDC-based screen-printed carbon electrode electrochemical immunosensors for detection of Hepatitis C virus
摘要
This work reports the modification of an Fe-BDC-based metal–organic framework using citric acid to be applied as a support material for a Hepatitis C virus immunosensor. Citric acid is believed to act as a capping agent that can control the growth of Fe-BDC particles. Fe-BDC was prepared using a solvothermal technique with varying amounts of citric acid. It was found that 0.3 mmol of citric acid produced Fe-BDC (Fe-BDC-CA3) with superior physicochemical properties, including small particles and high crystallinity. As an immunosensor, the Fe-BDC-modified screen-printed carbon electrode (SPCE) has a limit of detection (LOD) as low as 0.75 ng/mL and a broad linear detection range from 0 to 1000 ng/mL. The SPCE modified with Fe-BDC-CA3 also exhibited excellent selectivity and reproducibility. Additionally, the immunosensor showed good performance in detecting Hepatitis C antigen in human serum.