Scholarlink

<p>New approaches using high-throughput sequencing have described viral species in the gut microbiome or feces of marine mammals. In this study, we characterize a fraction of the virome using shotgun metagenomic analysis from two fresh fecal samples collected in a resting place from fur seals (<i>Arctocephalus gazella</i>) collected in the South Shetland Islands, Antarctica. Bacteria were predominant in the metagenomes, mainly represented by the phyla <i>Fusobacteria</i> (29.5%), <i>Proteobacteria</i> (23%), <i>Bacteroidetes</i> (22%), and <i>Firmicutes</i> (21%). We found 8,154 viral reads and, after de novo assembly, 81 viral contigs. Single-strand DNA viruses (ssDNA) were the most diverse among viral contigs (61.7%), followed by RNA viruses (29.6%) and double-strand DNA viruses (dsDNA) (8.6%), beyond detection of eleven viral families (<i>Anelloviridae, Bacciladnaviridae, Caliciviridae, Circoviridae, Guelinviridae, Kyanoviridae, Microviridae, Nodaviridae, Pachyviridae, Parvoviridae,</i> and <i>Picornaviridae</i>). Higher viral richness was observed for <i>Microviridae</i> family, and sequences closest related to viruses infecting invertebrates, fishes, and penguins could reveals a suggestive dietary pattern of Antarctic fur seals. Putative new Cyclovirus species was detected, beyond sequences related to Torque teno virus, Norovirus, and distinct members of <i>Picornavirales</i> Order. This study contributed to obtain a broader spectrum of fecal microbiome of this specific polar fauna.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Viral diversity in stool samples of fur seals from Antarctica using metagenomic shotgun

  • 期刊论文

  • 发表日期：

    2025年1月7日

查看全文
  • Tatiana Prado,
  • Daniel Andrade Moreira,
  • Maithê Gaspar Pontes Magalhães,
  • Letícia Ferreira Lima,
  • Martha Lima Brandão,
  • Marcia Chame,
  • Luciana Leomil,
  • Maria Ogrzewalska,
  • José Paulo Gagliardi Leite,
  • Wim Maurits Sylvain Degrave,
  • Marize Pereira Miagostovich,
  • Tulio Machado Fumian

摘要

New approaches using high-throughput sequencing have described viral species in the gut microbiome or feces of marine mammals. In this study, we characterize a fraction of the virome using shotgun metagenomic analysis from two fresh fecal samples collected in a resting place from fur seals (Arctocephalus gazella) collected in the South Shetland Islands, Antarctica. Bacteria were predominant in the metagenomes, mainly represented by the phyla Fusobacteria (29.5%), Proteobacteria (23%), Bacteroidetes (22%), and Firmicutes (21%). We found 8,154 viral reads and, after de novo assembly, 81 viral contigs. Single-strand DNA viruses (ssDNA) were the most diverse among viral contigs (61.7%), followed by RNA viruses (29.6%) and double-strand DNA viruses (dsDNA) (8.6%), beyond detection of eleven viral families (Anelloviridae, Bacciladnaviridae, Caliciviridae, Circoviridae, Guelinviridae, Kyanoviridae, Microviridae, Nodaviridae, Pachyviridae, Parvoviridae, and Picornaviridae). Higher viral richness was observed for Microviridae family, and sequences closest related to viruses infecting invertebrates, fishes, and penguins could reveals a suggestive dietary pattern of Antarctic fur seals. Putative new Cyclovirus species was detected, beyond sequences related to Torque teno virus, Norovirus, and distinct members of Picornavirales Order. This study contributed to obtain a broader spectrum of fecal microbiome of this specific polar fauna.