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Key message <p>Red light promotes <i>PsbS</i> transcription through the <i>PHYs</i>-<i>COP1</i> module, blue light through the <i>CRY1/CRY2-COP1</i> pathway, and UV-B via the <i>UVR8-COP1-HY5</i> cascade. ABA negatively regulates <i>PsbS</i> through the <i>PYLs-SnRK2s-ABI5</i> signaling pathway.</p> Abstract <p>Non-photochemical quenching (NPQ) is a central photoprotective mechanism that enables plants to dissipate excess excitation energy under high light conditions, with PsbS functioning as a key regulatory protein. However, the environmental cues controlling <i>PsbS</i> transcription and the underlying regulatory mechanisms remain largely unclear. Analysis of the core promoter region of <i>PsbS</i> identified light-responsive element G-box and abscisic acid-responsive element (ABRE) cis-elements; dual-luciferase reporter assays confirmed that the core <i>PsbS</i> promoter can respond to light and abscisic acid (ABA) signals. Expression analyses showed that red, blue, and ultraviolet-B (UV-B) light significantly induced <i>PsbS</i> transcription, whereas ABA treatment repressed it. Genetic and signaling analyses revealed that red light promotes <i>PsbS</i> transcription through the <i>PHYs</i>-<i>COP1</i> module, blue light through the <i>CRY1/CRY2-COP1</i> pathway, and UV-B via the <i>UVR8-COP1-HY5</i> cascade. In contrast, ABA negatively regulates <i>PsbS</i> through the <i>PYLs-SnRK2s-ABI5</i> signaling pathway. Yeast one-hybrid (Y1H) screening identified 36 candidate transcription factors targeting <i>PsbS</i>. Dual-luciferase assays and electrophoretic mobility shift assays (EMSA) further confirmed that bZIP2, bZIP16, bZIP43, CCA1, and IDD1 act as candidate transcriptional repressors of <i>PsbS</i>. This study systematically integrates known light and ABA signaling pathways that regulate <i>PsbS</i> expression, providing potential molecular targets for improving crop photosynthetic efficiency.</p>

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Light and ABA signaling are involved in transcriptional regulation of PsbS in plants

  • 期刊论文

  • 发表日期：

    2026年7月30日

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  • Peng Wang,
  • Shurui Yang,
  • Liankai Lv,
  • Mengyu Li,
  • Mengqi Lu,
  • Aihong Zhang,
  • Congming Lu

摘要

Key message

Red light promotes PsbS transcription through the PHYs-COP1 module, blue light through the CRY1/CRY2-COP1 pathway, and UV-B via the UVR8-COP1-HY5 cascade. ABA negatively regulates PsbS through the PYLs-SnRK2s-ABI5 signaling pathway.

Abstract

Non-photochemical quenching (NPQ) is a central photoprotective mechanism that enables plants to dissipate excess excitation energy under high light conditions, with PsbS functioning as a key regulatory protein. However, the environmental cues controlling PsbS transcription and the underlying regulatory mechanisms remain largely unclear. Analysis of the core promoter region of PsbS identified light-responsive element G-box and abscisic acid-responsive element (ABRE) cis-elements; dual-luciferase reporter assays confirmed that the core PsbS promoter can respond to light and abscisic acid (ABA) signals. Expression analyses showed that red, blue, and ultraviolet-B (UV-B) light significantly induced PsbS transcription, whereas ABA treatment repressed it. Genetic and signaling analyses revealed that red light promotes PsbS transcription through the PHYs-COP1 module, blue light through the CRY1/CRY2-COP1 pathway, and UV-B via the UVR8-COP1-HY5 cascade. In contrast, ABA negatively regulates PsbS through the PYLs-SnRK2s-ABI5 signaling pathway. Yeast one-hybrid (Y1H) screening identified 36 candidate transcription factors targeting PsbS. Dual-luciferase assays and electrophoretic mobility shift assays (EMSA) further confirmed that bZIP2, bZIP16, bZIP43, CCA1, and IDD1 act as candidate transcriptional repressors of PsbS. This study systematically integrates known light and ABA signaling pathways that regulate PsbS expression, providing potential molecular targets for improving crop photosynthetic efficiency.