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Key message <p>The KASP13 marker is located on chromosome 1 of <i>Cerasus humilis</i>. The AA genotype is associated with high acidity, whereas the AC genotype is associated with low acidity, with a prediction accuracy of 86.84%. This marker can be used to develop low-acid <i>C. humilis</i> cultivars.</p> Abstract <p>Common commercial cultivars of <i>Cerasus humilis</i> generally have high fruit acidity. Our research group previously constructed a hybrid of the ‘Jinou 1’ (high acidity) and ‘DS-1’ (low acidity) cultivars of <i>C. humilis</i>. Here, we conducted quantitative trait locus (QTL) mapping of fruit acidity using 208 F1 progeny of this cross to construct a high-density genetic map. We then used these findings to develop kompetitive allele-specific PCR (KASP) molecular markers. Results collected over 2 consecutive years showed that the hybrid population of ‘Jinou 1’ × ‘DS-1’ exhibited a continuous normal distribution of titratable acid content in their fruits, indicating that acidity level was a typical quantitative trait controlled by multiple genes. Stemming from this finding, interval mapping identified 16 QTLs associated with fruit acidity. The phenotypic variation contribution rates ranged from 8.6 to 25%. We then used KASP to develop molecular markers for organic acids, all of which were located to chromosome 1, with genotypes AA and AC representing the high- and low-acid types, respectively. KASP markers were used for genotype validation in 41 different acidic <i>C. humilis</i> varieties (lines) and 3 hybrid populations. This study provides a solid foundation for accelerated, efficient molecular-marker-assisted breeding of <i>C. humilis</i> cultivars which produce low-acidity fruits.</p>

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Development and application of KASP molecular marker for accelerated breeding of low-acid Cerasus humilis cultivars

  • 期刊论文

  • 发表日期：

    2026年7月3日

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  • Xia-Yu Xing,
  • Xiao-Yan Wang,
  • Yue-Zhan Xiao,
  • Hao-Nan Wang,
  • Hou-Qiang Song,
  • Ming Liu,
  • Xiao-Peng Mu,
  • Jian-Cheng Zhang,
  • Lu-Ting Jia,
  • Yu Gary Gao,
  • Shuai Zhang,
  • Peng-Fei Wang

摘要

Key message

The KASP13 marker is located on chromosome 1 of Cerasus humilis. The AA genotype is associated with high acidity, whereas the AC genotype is associated with low acidity, with a prediction accuracy of 86.84%. This marker can be used to develop low-acid C. humilis cultivars.

Abstract

Common commercial cultivars of Cerasus humilis generally have high fruit acidity. Our research group previously constructed a hybrid of the ‘Jinou 1’ (high acidity) and ‘DS-1’ (low acidity) cultivars of C. humilis. Here, we conducted quantitative trait locus (QTL) mapping of fruit acidity using 208 F1 progeny of this cross to construct a high-density genetic map. We then used these findings to develop kompetitive allele-specific PCR (KASP) molecular markers. Results collected over 2 consecutive years showed that the hybrid population of ‘Jinou 1’ × ‘DS-1’ exhibited a continuous normal distribution of titratable acid content in their fruits, indicating that acidity level was a typical quantitative trait controlled by multiple genes. Stemming from this finding, interval mapping identified 16 QTLs associated with fruit acidity. The phenotypic variation contribution rates ranged from 8.6 to 25%. We then used KASP to develop molecular markers for organic acids, all of which were located to chromosome 1, with genotypes AA and AC representing the high- and low-acid types, respectively. KASP markers were used for genotype validation in 41 different acidic C. humilis varieties (lines) and 3 hybrid populations. This study provides a solid foundation for accelerated, efficient molecular-marker-assisted breeding of C. humilis cultivars which produce low-acidity fruits.