Scholarlink

Key message <p>Endogenous constitutive promoters from <i>Nicotiana benthamiana</i> provide quantitatively defined transcriptional strengths across multiple species, enabling reliable alternatives to the CaMV 35S promoter for plant synthetic biology.</p> Abstract <p>In plant synthetic biology, precise control of gene expression requires constitutive promoters with quantitatively defined transcriptional strengths. Here, we identified endogenous constitutive promoter candidates from <i>Nicotiana benthamiana</i>. The transcriptional activities of the selected promoters were measured using GFP-based and dual-luciferase reporter assays in plants and benchmarked against the CaMV 35S promoter, revealing substantial variation in promoter strengths. Promoter activities were further evaluated in heterologous species, including <i>Brassica rapa</i>, <i>Capsicum annuum</i>, and <i>Zea mays</i>, using protoplast-based dual-luciferase assays. Cross-species analyses showed that relative promoter ranking was partially retained among dicot species, whereas transcriptional activity varied substantially depending on species and was generally reduced in maize. Together, this study defines a set of endogenous constitutive promoters across a range of transcriptional strengths, providing quantitative guidance for selecting endogenous alternatives to the CaMV 35S promoter in plant biotechnology and synthetic biology applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Endogenous constitutive promoters with a broad range of transcriptional activities for plant synthetic biology

  • 期刊论文

  • 发表日期：

    2026年6月22日

查看全文
  • Hyunjin Koo,
  • Sang Woo Lee,
  • Chae-Young Seo,
  • Minah Jung,
  • Dasom Choi,
  • Young Mi Shin,
  • Yerin Chang,
  • Hyun-Ah Lee,
  • Yong-Min Kim

摘要

Key message

Endogenous constitutive promoters from Nicotiana benthamiana provide quantitatively defined transcriptional strengths across multiple species, enabling reliable alternatives to the CaMV 35S promoter for plant synthetic biology.

Abstract

In plant synthetic biology, precise control of gene expression requires constitutive promoters with quantitatively defined transcriptional strengths. Here, we identified endogenous constitutive promoter candidates from Nicotiana benthamiana. The transcriptional activities of the selected promoters were measured using GFP-based and dual-luciferase reporter assays in plants and benchmarked against the CaMV 35S promoter, revealing substantial variation in promoter strengths. Promoter activities were further evaluated in heterologous species, including Brassica rapa, Capsicum annuum, and Zea mays, using protoplast-based dual-luciferase assays. Cross-species analyses showed that relative promoter ranking was partially retained among dicot species, whereas transcriptional activity varied substantially depending on species and was generally reduced in maize. Together, this study defines a set of endogenous constitutive promoters across a range of transcriptional strengths, providing quantitative guidance for selecting endogenous alternatives to the CaMV 35S promoter in plant biotechnology and synthetic biology applications.