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Key message <p>PagAPX8 enhances drought and salt tolerance in 84K poplar via boosting antioxidant enzyme activities to maintain ROS homeostasis, actingas a promising gene resource for stress-resistant woody plant breeding.</p> Abstract <p>Poplar (<i>Populus</i>) has been identified as a key species in the context of ecological restoration and reforestation. Nonetheless, abiotic stresses, including drought and salinization, impede growth by leading to a significant buildup of reactive oxygen species (ROS). Ascorbate peroxidase (APX) is an essential enzyme that plays a crucial role in scavenging ROS and improving stress resistance in plants. However, the specific roles of <i>APX</i> genes in poplars under stress conditions, such as drought and salinization, remain ambiguous. In this study, we examined 11 <i>PagAPX</i> genes in 84&#xa0;K poplar, and the overexpression of <i>PagAPX8</i> not only enhanced the growth of poplar but also increased the activity of the APX enzyme in poplar plants. In comparison with non-transgenic poplar plants (NT), <i>PagAPX8</i>-overexpressing poplars (<i>PagAPX8</i> OE) demonstrated enhanced tolerance to drought and salt stress, as indicated by increased fresh and dry weights, and superior root development. Physiological analysis revealed that <i>PagAPX8</i> OE plants exhibited significantly higher ROS-scavenging capacity, achieved by increased APX, superoxide dismutase (SOD), and catalase (CAT) activity, which resulted in a reduction of H₂O₂ and O₂⁻ accumulation in <i>PagAPX8</i> OE plants. Overall, this study indicated that <i>PagAPX8</i> improved drought and salt tolerance in poplar by regulating ROS homeostasis, thus underlining its potential as a valuable genetic resource for enhancing stress resistance in woody plants.</p>

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Overexpression of PagAPX8 promotes poplar growth and enhances tolerance to drought and salt stress

  • 期刊论文

  • 发表日期：

    2026年6月22日

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  • Yuanyuan Shang,
  • Zhaoyang Tian,
  • Yingxin Zhang,
  • Shuo Song,
  • Yu Guo,
  • Zibin Song,
  • Hongling Mo,
  • Kunlin Wang,
  • Yanqiu Zhao,
  • Hongxia Zhang,
  • Lizi Zhao

摘要

Key message

PagAPX8 enhances drought and salt tolerance in 84K poplar via boosting antioxidant enzyme activities to maintain ROS homeostasis, actingas a promising gene resource for stress-resistant woody plant breeding.

Abstract

Poplar (Populus) has been identified as a key species in the context of ecological restoration and reforestation. Nonetheless, abiotic stresses, including drought and salinization, impede growth by leading to a significant buildup of reactive oxygen species (ROS). Ascorbate peroxidase (APX) is an essential enzyme that plays a crucial role in scavenging ROS and improving stress resistance in plants. However, the specific roles of APX genes in poplars under stress conditions, such as drought and salinization, remain ambiguous. In this study, we examined 11 PagAPX genes in 84 K poplar, and the overexpression of PagAPX8 not only enhanced the growth of poplar but also increased the activity of the APX enzyme in poplar plants. In comparison with non-transgenic poplar plants (NT), PagAPX8-overexpressing poplars (PagAPX8 OE) demonstrated enhanced tolerance to drought and salt stress, as indicated by increased fresh and dry weights, and superior root development. Physiological analysis revealed that PagAPX8 OE plants exhibited significantly higher ROS-scavenging capacity, achieved by increased APX, superoxide dismutase (SOD), and catalase (CAT) activity, which resulted in a reduction of H₂O₂ and O₂⁻ accumulation in PagAPX8 OE plants. Overall, this study indicated that PagAPX8 improved drought and salt tolerance in poplar by regulating ROS homeostasis, thus underlining its potential as a valuable genetic resource for enhancing stress resistance in woody plants.