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Key message <p>Pepper CcSnRK2.5 confers drought adaptation, off ering a key genetic resource for crop improvement.</p> Abstract <p>Drought stress remains a paramount constraint on agricultural productivity, threatening global food security by impairing plant growth and inducing yield instability. As central mediators of plant stress adaptation, <i>sucrose non-fermenting 1-related protein kinase 2</i> (<i>SnRK2</i>) family members orchestrate complex abiotic stress signaling networks. In this study, we systematically identified <i>SnRK2</i> orthologs in pepper (<i>Capsicum chinense</i>), which were phylogenetically categorized into three distinct subgroups. Promoter analysis revealed a significant enrichment of ABA-responsive elements (ABREs) and drought-associated <i>cis</i>-regulatory motifs. Spatiotemporal expression profiling uncovered divergent transcript abundance patterns in leaves and roots under progressive drought stress, demonstrating organ-specific regulation of SnRK2-mediated stress perception. Functional validation via virus-induced gene silencing (VIGS) of <i>CcSnRK2.5</i> in pepper significantly augmented drought susceptibility, evidenced by increased oxidative damage and water loss. Conversely, heterologous overexpression of <i>CcSnRK2.5</i> in <i>Arabidopsis thaliana</i> enhanced drought tolerance by modulating the expression of key ABA-responsive marker genes. Collectively, these findings elucidate the regulatory role of SnRK2 genes in drought stress adaptation and provide promising genetic targets for the molecular breeding of drought-resilient pepper cultivars.</p>

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Molecular characterization of SnRK2 gene family in Capsicum chinense and functional validation of CcSnRK2.5 under drought stress

  • 期刊论文

  • 发表日期：

    2026年3月11日

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  • Huangying Shu,
  • Yu Zhang,
  • Jiancheng Liu,
  • Liping Zhang,
  • Xu Lu,
  • Zhoubin Liu,
  • Shanhan Cheng,
  • Huizhen Fu,
  • Zhiwei Wang

摘要

Key message

Pepper CcSnRK2.5 confers drought adaptation, off ering a key genetic resource for crop improvement.

Abstract

Drought stress remains a paramount constraint on agricultural productivity, threatening global food security by impairing plant growth and inducing yield instability. As central mediators of plant stress adaptation, sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members orchestrate complex abiotic stress signaling networks. In this study, we systematically identified SnRK2 orthologs in pepper (Capsicum chinense), which were phylogenetically categorized into three distinct subgroups. Promoter analysis revealed a significant enrichment of ABA-responsive elements (ABREs) and drought-associated cis-regulatory motifs. Spatiotemporal expression profiling uncovered divergent transcript abundance patterns in leaves and roots under progressive drought stress, demonstrating organ-specific regulation of SnRK2-mediated stress perception. Functional validation via virus-induced gene silencing (VIGS) of CcSnRK2.5 in pepper significantly augmented drought susceptibility, evidenced by increased oxidative damage and water loss. Conversely, heterologous overexpression of CcSnRK2.5 in Arabidopsis thaliana enhanced drought tolerance by modulating the expression of key ABA-responsive marker genes. Collectively, these findings elucidate the regulatory role of SnRK2 genes in drought stress adaptation and provide promising genetic targets for the molecular breeding of drought-resilient pepper cultivars.