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Key Message <p>Integrated host–pest proteomics revealed that chickpea resistance to <i>Helicoverpa armigera</i> involves early jasmonic acid signaling and linoleic acid–derived pro-toxins from crop wild relatives, providing molecular targets for breeding insect-resilient cultivars.</p> Abstract <p>Chickpea (<i>Cicer arietinum</i> L.), being a vital food legume, suffers severe yield reductions due to the pod borer <i>Helicoverpa armigera</i>. Despite extensive breeding efforts, durable resistance has remained elusive due to limited insights into the molecular basis of host–pest interactions. To address this gap, a first-of-its-kind integrated host–pest proteomic analysis in chickpea was performed to unravel the molecular mechanisms underlying natural insect resistance. Using untargeted LC–MS/MS, the proteomes resistant (ICCV506EB), susceptible (ICC3137) and a resistant crop wild relative (CWR’s) (IG73016, <i>C. cuneatum</i>), along with larvae feeding on these genotypes, were simultaneously profiled. Resistant genotypes elicited a rapid, multi-layered defense response involving jasmonic acid (JA)-mediated signaling, transcriptional reprogramming, and fatty acid–derived secondary metabolites. In turn, <i>H. armigera</i> activated detoxification enzymes, proteolytic modulation, and behavioral countermeasures. Strikingly, larvae feeding on resistant CWRs failed to overcome defenses, as linoleic acid (LA) derivatives are suggested to act as pro-toxin-like factors, adversely affecting larval survival, digestion, growth, and development. The findings reveal the dynamic defense–counter-defense interplay between chickpea and <i>H. armigera</i>. This interplay highlights the key biomolecular nodes associated with durable resistance. This study provides correlative evidence suggesting that LA-derived defense metabolites may function as potential pro-toxin-like compounds and establishes CWRs as a rich source of resistance traits. Importantly, enhancing early JA-pathway activation through molecular breeding or biotechnology could accelerate the development of insect-resilient chickpea cultivars, thereby boosting crop productivity and sustainability.</p>

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Comparative proteomics provides Insights into activation of jasmonic acid-mediated resistance mechanism in chickpea–Helicoverpa armigera interaction

  • 期刊论文

  • 发表日期：

    2026年3月10日

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  • Gothe Revanayya,
  • Inderjit Singh,
  • Onkarappa Dhanyakumar,
  • Jagdish Jaba,
  • Dharminder Bhatia,
  • Shayla Bindra,
  • Ravinder Singh,
  • Himabindu Kudapa,
  • Kalenahalli Yogendra

摘要

Key Message

Integrated host–pest proteomics revealed that chickpea resistance to Helicoverpa armigera involves early jasmonic acid signaling and linoleic acid–derived pro-toxins from crop wild relatives, providing molecular targets for breeding insect-resilient cultivars.

Abstract

Chickpea (Cicer arietinum L.), being a vital food legume, suffers severe yield reductions due to the pod borer Helicoverpa armigera. Despite extensive breeding efforts, durable resistance has remained elusive due to limited insights into the molecular basis of host–pest interactions. To address this gap, a first-of-its-kind integrated host–pest proteomic analysis in chickpea was performed to unravel the molecular mechanisms underlying natural insect resistance. Using untargeted LC–MS/MS, the proteomes resistant (ICCV506EB), susceptible (ICC3137) and a resistant crop wild relative (CWR’s) (IG73016, C. cuneatum), along with larvae feeding on these genotypes, were simultaneously profiled. Resistant genotypes elicited a rapid, multi-layered defense response involving jasmonic acid (JA)-mediated signaling, transcriptional reprogramming, and fatty acid–derived secondary metabolites. In turn, H. armigera activated detoxification enzymes, proteolytic modulation, and behavioral countermeasures. Strikingly, larvae feeding on resistant CWRs failed to overcome defenses, as linoleic acid (LA) derivatives are suggested to act as pro-toxin-like factors, adversely affecting larval survival, digestion, growth, and development. The findings reveal the dynamic defense–counter-defense interplay between chickpea and H. armigera. This interplay highlights the key biomolecular nodes associated with durable resistance. This study provides correlative evidence suggesting that LA-derived defense metabolites may function as potential pro-toxin-like compounds and establishes CWRs as a rich source of resistance traits. Importantly, enhancing early JA-pathway activation through molecular breeding or biotechnology could accelerate the development of insect-resilient chickpea cultivars, thereby boosting crop productivity and sustainability.