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Key message <p>The bHLH transcription factor BoMYC2 played a critical role in regulating branching in ornamental kale via the BoMYC2-<i>BoD27</i> cascade by transcriptionally repressing<i> BoD27</i> to reduce strigolactone (SL) biosynthesis and promote branching.</p> Abstract <p>Branching plays a vital role in plant morphology. Ornamental kale is an emerging cold-season flower, branching is of great significance on its ornamental value. While the functional genes and corresponding mechanism of branching in ornamental kale remains unclear. Previously, we conducted transcriptome sequencing with a single-branched inbred line ‘P29’ and its multi-branched mutant<i>.</i> Among the differentially expressed genes, <i>BoD27,</i> a homologous gene of <i>AtD27</i>, was a candidate. In this study, we cloned the full-length and promoter sequences of the <i>BoD27</i> gene between the single- and the multi-branched materials<i>.</i> The differences within the coding sequences led to eleven amino acids mutations, where three mutated amino acids located in the functional domain. The BoD27 protein localized in chloroplast under the laser scanning confocal microscope. Knockout and knockdown of the <i>BoD27</i> gene both led to SLs content decrease and promoted the outgrowth of axillary buds. BoMYC2 was identified as an upstream inhibitory factor of <i>BoD27</i> gene via Y1H and DLR assays.&#xa0;Overexpression of BoMYC2 promoted the numbers of branches by inhibiting Sls synthesis. This study revealed a novel regulatory cascade comprising <i>BoD27</i> and its upstream inhibitor BoMYC2 that regulates branching in ornamental kale.</p>

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The BoMYC2-BoD27 cascade regulates branching by influencing the content of strigolactones in ornamental kale

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Rui Liu,
  • Xin Feng,
  • Yang Liu,
  • Yujia Dai,
  • Xiyuan Yin,
  • Pengfang Zhu

摘要

Key message

The bHLH transcription factor BoMYC2 played a critical role in regulating branching in ornamental kale via the BoMYC2-BoD27 cascade by transcriptionally repressing BoD27 to reduce strigolactone (SL) biosynthesis and promote branching.

Abstract

Branching plays a vital role in plant morphology. Ornamental kale is an emerging cold-season flower, branching is of great significance on its ornamental value. While the functional genes and corresponding mechanism of branching in ornamental kale remains unclear. Previously, we conducted transcriptome sequencing with a single-branched inbred line ‘P29’ and its multi-branched mutant. Among the differentially expressed genes, BoD27, a homologous gene of AtD27, was a candidate. In this study, we cloned the full-length and promoter sequences of the BoD27 gene between the single- and the multi-branched materials. The differences within the coding sequences led to eleven amino acids mutations, where three mutated amino acids located in the functional domain. The BoD27 protein localized in chloroplast under the laser scanning confocal microscope. Knockout and knockdown of the BoD27 gene both led to SLs content decrease and promoted the outgrowth of axillary buds. BoMYC2 was identified as an upstream inhibitory factor of BoD27 gene via Y1H and DLR assays. Overexpression of BoMYC2 promoted the numbers of branches by inhibiting Sls synthesis. This study revealed a novel regulatory cascade comprising BoD27 and its upstream inhibitor BoMYC2 that regulates branching in ornamental kale.