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<p>MYB transcription factors play a pivotal role in plant responses to cold stress. However, the underlying regulatory mechanisms in eggplant (<i>Solanum melongena</i>) remain unclear. In our previous study, based on transcriptomic analysis, we identified a MYB transcription factor as a core regulatory gene involved in cold stress responses in eggplant. In this study, we isolated and cloned <i>SmMYB88</i> from eggplant. SmMYB88 was localized to the nucleus and exhibited transactivation activity. Heterologous overexpression of SmMYB88 in <i>Arabidopsis thaliana</i> enhanced the plant’s cold tolerance. SmMYB88 specifically bound to the promote elements of <i>SmGST</i> and <i>SmGPX</i>. Furthermore, the interaction between SmMYB88 and SmERF1 further up-regulated the expression of <i>SmGST</i> and <i>SmGPX</i> genes. The up-regulated <i>SmGST</i> and <i>SmGPX</i> enhanced the plant's capacity to scavenge reactive oxygen species (ROS) by increasing glutathione metabolism and corresponding enzyme activities, thereby improving the plant’s tolerance to cold stress. Additionally, overexpression of <i>SmMYB88</i> significantly increased the expression levels of <i>ICE1</i>, <i>CBFs</i>, and <i>COR47</i> in <i>Arabidopsis</i> (<i>p</i> &lt; 0.05), suggesting that <i>SmMYB88</i> may enhance plant cold tolerance by regulating the expression of CBF network genes. Therefore, this gene may serve as a potential target for genetic engineering to improve cold tolerance in eggplant.</p>

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A R2R3-MYB transcription factor SmMYB88 of Solanum melongena regulates cold tolerance by activating antioxidant system

  • 期刊论文

  • 发表日期：

    2026年1月14日

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  • Yanhong Lan,
  • Fangyi Gong,
  • Rongjiang Dong,
  • Chun Li,
  • Feng Xia,
  • Yifan Li,
  • Xiaojun Liu,
  • Duchen Liu,
  • Genyun Liang,
  • Chao Fang,
  • Peng Cai

摘要

MYB transcription factors play a pivotal role in plant responses to cold stress. However, the underlying regulatory mechanisms in eggplant (Solanum melongena) remain unclear. In our previous study, based on transcriptomic analysis, we identified a MYB transcription factor as a core regulatory gene involved in cold stress responses in eggplant. In this study, we isolated and cloned SmMYB88 from eggplant. SmMYB88 was localized to the nucleus and exhibited transactivation activity. Heterologous overexpression of SmMYB88 in Arabidopsis thaliana enhanced the plant’s cold tolerance. SmMYB88 specifically bound to the promote elements of SmGST and SmGPX. Furthermore, the interaction between SmMYB88 and SmERF1 further up-regulated the expression of SmGST and SmGPX genes. The up-regulated SmGST and SmGPX enhanced the plant's capacity to scavenge reactive oxygen species (ROS) by increasing glutathione metabolism and corresponding enzyme activities, thereby improving the plant’s tolerance to cold stress. Additionally, overexpression of SmMYB88 significantly increased the expression levels of ICE1, CBFs, and COR47 in Arabidopsis (p < 0.05), suggesting that SmMYB88 may enhance plant cold tolerance by regulating the expression of CBF network genes. Therefore, this gene may serve as a potential target for genetic engineering to improve cold tolerance in eggplant.