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Key message <p>Functional analysis in <i>Arabidopsis</i> revealed that <i>ZjRVE8 -1</i> significantly enhances cold tolerance by activating the CBF pathway, identifying it as a promising gene for breeding cold hardy <i>Zoysia japonica</i> cultivars.</p> Abstract <p>REVEILLE (RVE) transcription factors regulate circadian rhythms and abiotic stress responses, but their roles in cold tolerance in the warm-season turfgrass <i>Zoysia japonica</i> remain uncharacterized. We identified 10 RVE family members through genome-wide analysis and performed comprehensive expression profiling under multiple abiotic stresses, revealing cold-responsive patterns. Based on this, five cold-induced <i>RVE</i> genes were selected for functional validation via heterologous overexpression in <i>Arabidopsis thaliana</i>. Overexpression of <i>ZjRVE8-1</i> significantly enhanced freezing tolerance under both non-acclimated (NA) and cold-acclimated (CA) conditions by upregulating <i>CBF1</i> and <i>CBF3</i> expression. In contrast, overexpression of four tested RVE1-clade members failed to improve, and in one case reduced, freezing tolerance, correlating with their divergent cold-induced expression patterns. Promoter analysis identified a low-temperature responsive (LTR) element exclusively in <i>ZjRVE8-1</i> of the cold-tolerant genotype, explaining its superior induction. This study uncovers <i>ZjRVE8-1</i> as a novel regulator of cold adaptation via <i>CBF</i> activation and provides functional insights into RVE family divergence, offering candidate genes for breeding cold-hardy <i>Z. japonica</i>.</p>

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Genome-wide analysis of Zoysia japonica REVEILLE family identifies ZjRVE8-1 as a positive cold-tolerance regulator

  • 期刊论文

  • 发表日期：

    2025年12月26日

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  • Cuiling Liu,
  • Liangliang He,
  • Ming Jiang,
  • Zhihao Wu,
  • Jieyi Li,
  • Chao Liu,
  • Quanrui Su,
  • Jingyi Sun,
  • Fei Xu,
  • Shu Chen

摘要

Key message

Functional analysis in Arabidopsis revealed that ZjRVE8 -1 significantly enhances cold tolerance by activating the CBF pathway, identifying it as a promising gene for breeding cold hardy Zoysia japonica cultivars.

Abstract

REVEILLE (RVE) transcription factors regulate circadian rhythms and abiotic stress responses, but their roles in cold tolerance in the warm-season turfgrass Zoysia japonica remain uncharacterized. We identified 10 RVE family members through genome-wide analysis and performed comprehensive expression profiling under multiple abiotic stresses, revealing cold-responsive patterns. Based on this, five cold-induced RVE genes were selected for functional validation via heterologous overexpression in Arabidopsis thaliana. Overexpression of ZjRVE8-1 significantly enhanced freezing tolerance under both non-acclimated (NA) and cold-acclimated (CA) conditions by upregulating CBF1 and CBF3 expression. In contrast, overexpression of four tested RVE1-clade members failed to improve, and in one case reduced, freezing tolerance, correlating with their divergent cold-induced expression patterns. Promoter analysis identified a low-temperature responsive (LTR) element exclusively in ZjRVE8-1 of the cold-tolerant genotype, explaining its superior induction. This study uncovers ZjRVE8-1 as a novel regulator of cold adaptation via CBF activation and provides functional insights into RVE family divergence, offering candidate genes for breeding cold-hardy Z. japonica.