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Key message <p><i>NtRPP13</i>, a CNL-type gene suppressed by <i>Ralstonia solanacearum</i>, mediates a positive regulation of hypersensitive response and phytohormone-related defense genes for bacterial wilt resistance in tobacco.</p> Abstract <p>Bacterial wilt, caused by <i>Ralstonia solanacearum</i>, is a devastating soil-borne disease that infects hundreds of plant species worldwide. To date, while effective control strategies for this pathogen remain limited, exploring resistant genes is particularly important in disease-resistant breeding. Nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins are key participants in effector-triggered immunity in plants. This study identified a novel NBS-LRR resistance gene, <i>NtRPP13</i>, in tobacco, which exhibited downregulation in roots of a susceptible tobacco cultivar upon <i>R. solanacearum</i> infection. The NtRPP13 protein contained a typical coiled-coil (CC) domain at its N-terminus and was classified into the CC-NBS-LRR category. Subcellular localization analysis revealed that NtRPP13 localizes to the plasma membrane. Additionally, exposure to phytohormones—including abscisic acid, auxin and gibberellic acid, and abiotic stressors such as drought and cold altered <i>NtRPP13</i> expression. This could be attribute to the corresponding <i>cis</i>-acting elements in the <i>NtRPP13</i> promoter. Transient overexpression of <i>NtRPP13</i> triggered a hypersensitive response (HR) in <i>Nicotiana benthamiana</i>, while stable overexpression in transgenic tobacco plants significantly enhanced resistance to <i>R. solanacearum</i>, with varying resistance levels observed between different transgenic lines. Moreover, following inoculation with <i>R. solanacearum</i>, the transgenic plants exhibited marked upregulation of some key defense-related marker genes associated with the HR, salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways, along with significantly elevated levels of JA and SA, compared to wild-type controls. These findings suggest that <i>NtRPP13</i> contributes to tobacco defense against <i>R. solanacearum</i> by mediating crosstalk between multiple signaling pathways.</p>

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Overexpression of a novel CC-NBS-LRR gene NtRPP13 confers enhanced resistance to Ralstonia solanacearum in tobacco

  • 期刊论文

  • 发表日期：

    2025年11月21日

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  • Wen Yu,
  • Yazhi Cheng,
  • Jiale Jiang,
  • Zhijie Li,
  • Qingyun Li,
  • Weifeng Wang,
  • Zhixiao Yang,
  • Shengxin Wu,
  • Anming Ding

摘要

Key message

NtRPP13, a CNL-type gene suppressed by Ralstonia solanacearum, mediates a positive regulation of hypersensitive response and phytohormone-related defense genes for bacterial wilt resistance in tobacco.

Abstract

Bacterial wilt, caused by Ralstonia solanacearum, is a devastating soil-borne disease that infects hundreds of plant species worldwide. To date, while effective control strategies for this pathogen remain limited, exploring resistant genes is particularly important in disease-resistant breeding. Nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins are key participants in effector-triggered immunity in plants. This study identified a novel NBS-LRR resistance gene, NtRPP13, in tobacco, which exhibited downregulation in roots of a susceptible tobacco cultivar upon R. solanacearum infection. The NtRPP13 protein contained a typical coiled-coil (CC) domain at its N-terminus and was classified into the CC-NBS-LRR category. Subcellular localization analysis revealed that NtRPP13 localizes to the plasma membrane. Additionally, exposure to phytohormones—including abscisic acid, auxin and gibberellic acid, and abiotic stressors such as drought and cold altered NtRPP13 expression. This could be attribute to the corresponding cis-acting elements in the NtRPP13 promoter. Transient overexpression of NtRPP13 triggered a hypersensitive response (HR) in Nicotiana benthamiana, while stable overexpression in transgenic tobacco plants significantly enhanced resistance to R. solanacearum, with varying resistance levels observed between different transgenic lines. Moreover, following inoculation with R. solanacearum, the transgenic plants exhibited marked upregulation of some key defense-related marker genes associated with the HR, salicylic acid (SA), jasmonic acid (JA), and ethylene signaling pathways, along with significantly elevated levels of JA and SA, compared to wild-type controls. These findings suggest that NtRPP13 contributes to tobacco defense against R. solanacearum by mediating crosstalk between multiple signaling pathways.