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Key message <p>In this research, we identified the important role of CsMYB-CC11 in responding to drought stress and confirmed its downstream target gene CsPIP2;4.</p> Abstract <p>MYB-CC are members of the MYB transcription factors (TFs) superfamily, which is characterized by the inclusion of a conserved MYB DNA-binding structural domain and a coiled-coil structural domain. Many of the functions of the MYB-CC family have been reported in Arabidopsis, rice, and tomato, but systematic studies in cucumber are lacking. Through genome-wide characterization, thirteen <i>CsMYB-CC</i> family genes were identified across cucumber, with cis-acting element analysis of their promoter regions revealing an enrichment of motifs associated with both developmental processes and stress response pathways. Phylogenetic and synteny analyses demonstrated evolutionary conservation of MYB-CC proteins among diverse plant species. Transcriptional profiling utilizing existing RNA-seq datasets combined with quantitative reverse transcription PCR (qRT-PCR) validation revealed differential expression patterns of <i>CsMYB-CC</i> genes under various treatment conditions. Further studies confirmed that CsMYB-CC11 was localized in the nucleus and acted as a transcriptional repressor to negatively regulate the expression of <i>CsPIP2;4</i>. The role of <i>CsMYB-CC11</i> in drought stress response was investigated through transient overexpression and virus-induced gene silencing (VIGS) experiments. Overexpression of <i>CsMYB-CC11</i> reduced drought tolerance in plants, whereas VIGS silencing of <i>CsMYB-CC11</i> enhanced tolerance, such as greener leaves, higher chlorophyll content, and maintenance of stronger antioxidant enzyme activities. This investigation establishes an essential foundation study focusing on the role of MYB-CC TF in cucumber stress adaptation mechanisms.</p>

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Genome-wide identification unravels the role of the MYB-CC family in cucumber stress response

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Yang Yang,
  • Lufeng Zheng,
  • Yutian Zhang,
  • Changxia Du,
  • Huaifu Fan

摘要

Key message

In this research, we identified the important role of CsMYB-CC11 in responding to drought stress and confirmed its downstream target gene CsPIP2;4.

Abstract

MYB-CC are members of the MYB transcription factors (TFs) superfamily, which is characterized by the inclusion of a conserved MYB DNA-binding structural domain and a coiled-coil structural domain. Many of the functions of the MYB-CC family have been reported in Arabidopsis, rice, and tomato, but systematic studies in cucumber are lacking. Through genome-wide characterization, thirteen CsMYB-CC family genes were identified across cucumber, with cis-acting element analysis of their promoter regions revealing an enrichment of motifs associated with both developmental processes and stress response pathways. Phylogenetic and synteny analyses demonstrated evolutionary conservation of MYB-CC proteins among diverse plant species. Transcriptional profiling utilizing existing RNA-seq datasets combined with quantitative reverse transcription PCR (qRT-PCR) validation revealed differential expression patterns of CsMYB-CC genes under various treatment conditions. Further studies confirmed that CsMYB-CC11 was localized in the nucleus and acted as a transcriptional repressor to negatively regulate the expression of CsPIP2;4. The role of CsMYB-CC11 in drought stress response was investigated through transient overexpression and virus-induced gene silencing (VIGS) experiments. Overexpression of CsMYB-CC11 reduced drought tolerance in plants, whereas VIGS silencing of CsMYB-CC11 enhanced tolerance, such as greener leaves, higher chlorophyll content, and maintenance of stronger antioxidant enzyme activities. This investigation establishes an essential foundation study focusing on the role of MYB-CC TF in cucumber stress adaptation mechanisms.