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Key message <p>The receptor-like kinase SlLRR-RLK94 enhances tomato resistance to <i>Phytophthora infestans</i> by effecting pathogenesis-related (<i>PR</i>) gene expression, reactive oxygen species (ROS) homeostasis, and the phenylpropanoid biosynthesis pathway.</p> Abstract <p>Late blight, caused by <i>Phytophthora infestans</i> (<i>P. infestans</i>), is one of the most devastating diseases affecting tomato yield and quality. Receptor-like kinases (RLKs) are essential for plants to sense various signaling molecules and to trigger early immune responses. However, most Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest RLK subfamily, have unknown functions in tomato resistance to <i>P. infestans</i>. Here, we identified 209 LRR-RLK family members in tomato, and clustered them into 14 subfamilies based on phylogenetic analysis. Transcriptome analysis revealed that <i>SlLRR-RLK94</i> (belonging to the XI subfamily) showed the strongest response to <i>P. infestans</i> infection. Using virus-induced gene silencing and overexpression in tomato, we demonstrated that <i>SlLRR-RLK94</i> positively regulates tomato resistance to <i>P. infestans.</i> In addition, <i>SlLRR-RLK94</i> also regulates the expression of <i>PR</i> genes, ROS-scavenging genes, and antioxidant enzyme activity. Integrated transcriptomic and metabolomic analyses suggested that <i>SlLRR-RLK94</i> mediates tomato resistance to <i>P. infestans</i>, possibly by effecting the phenylpropanoid biosynthesis pathway. These findings establish <i>SlLRR-RLK94</i> as a key factor facilitating tomato defense signaling and offer a valuable genetic basis for its potential application in crop breeding.</p>

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The receptor-like kinase SlLRR-RLK94 as a positive regulator of tomato resistance to Phytophthora infestans

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • Jiaxuan Zhu,
  • Yan Li,
  • Ruirui Yang,
  • Zhiyuan Xue,
  • Ruili Lv,
  • Hongbo Wei,
  • Zhengjie Wang,
  • Ruiming Wang,
  • Yushi Luan

摘要

Key message

The receptor-like kinase SlLRR-RLK94 enhances tomato resistance to Phytophthora infestans by effecting pathogenesis-related (PR) gene expression, reactive oxygen species (ROS) homeostasis, and the phenylpropanoid biosynthesis pathway.

Abstract

Late blight, caused by Phytophthora infestans (P. infestans), is one of the most devastating diseases affecting tomato yield and quality. Receptor-like kinases (RLKs) are essential for plants to sense various signaling molecules and to trigger early immune responses. However, most Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest RLK subfamily, have unknown functions in tomato resistance to P. infestans. Here, we identified 209 LRR-RLK family members in tomato, and clustered them into 14 subfamilies based on phylogenetic analysis. Transcriptome analysis revealed that SlLRR-RLK94 (belonging to the XI subfamily) showed the strongest response to P. infestans infection. Using virus-induced gene silencing and overexpression in tomato, we demonstrated that SlLRR-RLK94 positively regulates tomato resistance to P. infestans. In addition, SlLRR-RLK94 also regulates the expression of PR genes, ROS-scavenging genes, and antioxidant enzyme activity. Integrated transcriptomic and metabolomic analyses suggested that SlLRR-RLK94 mediates tomato resistance to P. infestans, possibly by effecting the phenylpropanoid biosynthesis pathway. These findings establish SlLRR-RLK94 as a key factor facilitating tomato defense signaling and offer a valuable genetic basis for its potential application in crop breeding.