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Key message <p>WGCNA mined the unknown gene <i>NtLYK5</i>, and VIGS and RNA-seq analyses suggested that <i>NtLYK5</i> mediates the negative regulation of hydrogen peroxide production for drought resistance.</p> Abstract <p>Drought during the seedling stage of tobacco (<i>Nicotiana tabacum</i>), a water-sensitive and economically important crop, has serious adverse effects on transplant survival and tobacco plant growth. In this study, we conducted transcriptome sequencing on drought-tolerant and drought-sensitive recombinant inbred lines (RILs) from the F7 generation of the cross “NC82 × Bina No. 1.” Using weighted gene co-expression network analysis (WGCNA), BLAST alignment, bioinformatics analysis, and qRT-PCR, we identified a key candidate gene <i>NtLYK5</i>, which is highly associated with drought resistance but previously had an unknown function. Virus-induced gene silencing to suppress <i>NtLYK5</i> expression in tobacco enhanced drought resistance, reduced hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels, and increased catalase (CAT) activity. Further transcriptomic analysis of drought-tolerant and drought-sensitive lines revealed differentially expressed genes (DEGs) associated with <i>NtLYK5</i>-induced drought resistance<i>,</i> which were significantly enriched in the MAPK signaling pathway. Under drought stress, H<sub>2</sub>O<sub>2</sub> acts as a signaling molecule affecting the expression of DEGs such as <i>ANP1</i>, which induces H<sub>2</sub>O<sub>2</sub> production and cell death, thereby preventing the stomata from closing properly. These results suggest that <i>NtLYK5</i> is a key gene that negatively regulates drought tolerance in tobacco seedlings by modulating H<sub>2</sub>O<sub>2</sub>-induced stomatal movement.</p>

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NtLYK5 negatively regulates drought resistance in tobacco through H2O2-mediated leaf stomatal opening and closing

  • 期刊论文

  • 发表日期：

    2025年8月29日

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  • Yonglu Xue,
  • Qiwei Yu,
  • Gang Chen,
  • Zhenbao Luo,
  • Kai Pi,
  • Baolin Xie,
  • Kaiping Wu,
  • Chenhao Shi,
  • Renxiang Liu

摘要

Key message

WGCNA mined the unknown gene NtLYK5, and VIGS and RNA-seq analyses suggested that NtLYK5 mediates the negative regulation of hydrogen peroxide production for drought resistance.

Abstract

Drought during the seedling stage of tobacco (Nicotiana tabacum), a water-sensitive and economically important crop, has serious adverse effects on transplant survival and tobacco plant growth. In this study, we conducted transcriptome sequencing on drought-tolerant and drought-sensitive recombinant inbred lines (RILs) from the F7 generation of the cross “NC82 × Bina No. 1.” Using weighted gene co-expression network analysis (WGCNA), BLAST alignment, bioinformatics analysis, and qRT-PCR, we identified a key candidate gene NtLYK5, which is highly associated with drought resistance but previously had an unknown function. Virus-induced gene silencing to suppress NtLYK5 expression in tobacco enhanced drought resistance, reduced hydrogen peroxide (H2O2) levels, and increased catalase (CAT) activity. Further transcriptomic analysis of drought-tolerant and drought-sensitive lines revealed differentially expressed genes (DEGs) associated with NtLYK5-induced drought resistance, which were significantly enriched in the MAPK signaling pathway. Under drought stress, H2O2 acts as a signaling molecule affecting the expression of DEGs such as ANP1, which induces H2O2 production and cell death, thereby preventing the stomata from closing properly. These results suggest that NtLYK5 is a key gene that negatively regulates drought tolerance in tobacco seedlings by modulating H2O2-induced stomatal movement.