Scholarlink

Key message <p>Antioxidant-related miRNA–mRNA pairs play crucial roles in modulating the dominant embryo formation in<i> Taxodium </i>hybrid ‘zhongshanshan’.</p> Abstract <p>SE (somatic embryogenesis) has been instrumental in the successful breeding of <i>Taxodium</i> hybrid ‘zhongshanshan’, a valuable timber tree species of great ecological importance. However, the underlying molecular mechanisms remain largely unknown. By examining the transcriptome and small RNA throughout the entire SE process, we observed notable differences in gene expression and miRNA profiles. DEGs (differentially expressed genes) associated with phenylpropanoid biosynthesis, glutathione metabolism, starch and sucrose metabolism, and pentose and glucuronate interconversions played pivotal roles in dominant embryo formation and cotyledonary embryo maturation. The physiological measurements showed that the contents of organic compounds were higher in embryogenic callus and cotyledonary embryo stage. Endogenous GSH (glutathione) and GSSG (oxidized glutathione disulfide) contents reached the peak values in the dominant embryo stage. Additionally, a total of 271 mature miRNAs were detected, with 244 being previously identified and 27 being novel plant miRNAs. Among these, 71 DE miRNAs (differentially expressed miRNAs) were screened out during the transition from embryogenic callus to cotyledon embryo stage of SE, which suggested that miRNAs played important roles in somatic embryo development. By predicting target genes for these DE miRNAs and conducting correlation analysis with DEGs, several key miRNA-mRNA pairs were identified, which mainly regulated the dominant embryo formation in <i>Taxodium</i> hybrid ‘zhongshanshan’. Furthermore, the exogenous addition of 0.3&#xa0;g·L<sup>−1</sup> GSH effectively improved SE efficiency. Taken together, this study provided valuable information in the SE of <i>Taxodium</i> hybrid ‘zhongshanshan’, thereby facilitating its breeding programs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptome and small RNA analysis reveal potential miRNA-mediated interactions involved in somatic embryogenesis of Taxodium hybrid ‘zhongshanshan’

  • 期刊论文

  • 发表日期：

    2025年8月4日

查看全文
  • Tingting Chen,
  • Guoying Yuan,
  • Yan Lu,
  • Dan Wang,
  • Haoran Qi,
  • Xiaowei Sun,
  • Jianfeng Hua,
  • Yunlong Yin,
  • Chaoguang Yu

摘要

Key message

Antioxidant-related miRNA–mRNA pairs play crucial roles in modulating the dominant embryo formation in Taxodium hybrid ‘zhongshanshan’.

Abstract

SE (somatic embryogenesis) has been instrumental in the successful breeding of Taxodium hybrid ‘zhongshanshan’, a valuable timber tree species of great ecological importance. However, the underlying molecular mechanisms remain largely unknown. By examining the transcriptome and small RNA throughout the entire SE process, we observed notable differences in gene expression and miRNA profiles. DEGs (differentially expressed genes) associated with phenylpropanoid biosynthesis, glutathione metabolism, starch and sucrose metabolism, and pentose and glucuronate interconversions played pivotal roles in dominant embryo formation and cotyledonary embryo maturation. The physiological measurements showed that the contents of organic compounds were higher in embryogenic callus and cotyledonary embryo stage. Endogenous GSH (glutathione) and GSSG (oxidized glutathione disulfide) contents reached the peak values in the dominant embryo stage. Additionally, a total of 271 mature miRNAs were detected, with 244 being previously identified and 27 being novel plant miRNAs. Among these, 71 DE miRNAs (differentially expressed miRNAs) were screened out during the transition from embryogenic callus to cotyledon embryo stage of SE, which suggested that miRNAs played important roles in somatic embryo development. By predicting target genes for these DE miRNAs and conducting correlation analysis with DEGs, several key miRNA-mRNA pairs were identified, which mainly regulated the dominant embryo formation in Taxodium hybrid ‘zhongshanshan’. Furthermore, the exogenous addition of 0.3 g·L−1 GSH effectively improved SE efficiency. Taken together, this study provided valuable information in the SE of Taxodium hybrid ‘zhongshanshan’, thereby facilitating its breeding programs.