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Key message <p><i>OsUGT72F1</i> enhances heat tolerance in plants by improving ROS scavenging and modifying multiple metabolic pathways, under the regulation of transcription factors OsHSFA3 and OsHSFA4a.</p> Abstract <p>High temperature is one of the most critical environmental constraints affecting plant growth and development, ultimately leading to yield losses in crops such as rice (<i>Oryza sativa</i> L.). UDP (uridine diphosphate)-dependent glycosyltransferases (UGTs) are believed to play crucial roles in coping with environmental stresses. However, the functions for the vast majority of UGTs under high temperature stress remain largely unknown. In this study, we isolated and characterized a high temperature responsive UDP-glycosyltransferase gene <i>OsUGT72F1</i> in rice. Our findings demonstrated that overexpression of <i>OsUGT72F1</i> enhanced heat-stress tolerance, while the mutant plants displayed a sensitive phenotype under the same stress conditions. Ectopic expression of <i>OsUGT72F1</i> in <i>Arabidopsis thaliana</i> also conferred improved heat tolerance to the plants. Further investigation revealed that <i>OsUGT72F1</i> reduced the generation of reactive oxygen species (ROS) and boosted the activity of antioxidant enzymes, thereby alleviating&#xa0;oxidative&#xa0;damage under heat-stress conditions. Moreover, transcriptomic analysis indicated that the action of <i>OsUGT72F1</i> leads to the upregulation of multiple metabolic pathways including phenylpropanoid biosynthesis, zeatin biosynthesis, and flavonoid biosynthesis. In addition, the upstream regulatory mechanism of the <i>OsUGT72F1</i> gene has been identified. We found that the transcription factors OsHSFA3 and OsHSFA4a can bind to the <i>OsUGT72F1</i> promoter and enhance its transcription level. Together, this study revealed that the glycosyltransferase gene <i>OsUGT72F1</i> plays a vital role in the adaptive adjustment of high temperature stress in plants, revealing a new heat tolerance pathway and providing a promising gene candidate for the breeding of heat-resistant crop varieties.</p>

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A high temperature responsive UDP-glucosyltransferase gene OsUGT72F1 enhances heat tolerance in rice and Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年2月3日

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  • Yuqing Ma,
  • Shuman Zhao,
  • Xinmei Ma,
  • Guangrui Dong,
  • Chonglin Liu,
  • Yi Ding,
  • Bingkai Hou

摘要

Key message

OsUGT72F1 enhances heat tolerance in plants by improving ROS scavenging and modifying multiple metabolic pathways, under the regulation of transcription factors OsHSFA3 and OsHSFA4a.

Abstract

High temperature is one of the most critical environmental constraints affecting plant growth and development, ultimately leading to yield losses in crops such as rice (Oryza sativa L.). UDP (uridine diphosphate)-dependent glycosyltransferases (UGTs) are believed to play crucial roles in coping with environmental stresses. However, the functions for the vast majority of UGTs under high temperature stress remain largely unknown. In this study, we isolated and characterized a high temperature responsive UDP-glycosyltransferase gene OsUGT72F1 in rice. Our findings demonstrated that overexpression of OsUGT72F1 enhanced heat-stress tolerance, while the mutant plants displayed a sensitive phenotype under the same stress conditions. Ectopic expression of OsUGT72F1 in Arabidopsis thaliana also conferred improved heat tolerance to the plants. Further investigation revealed that OsUGT72F1 reduced the generation of reactive oxygen species (ROS) and boosted the activity of antioxidant enzymes, thereby alleviating oxidative damage under heat-stress conditions. Moreover, transcriptomic analysis indicated that the action of OsUGT72F1 leads to the upregulation of multiple metabolic pathways including phenylpropanoid biosynthesis, zeatin biosynthesis, and flavonoid biosynthesis. In addition, the upstream regulatory mechanism of the OsUGT72F1 gene has been identified. We found that the transcription factors OsHSFA3 and OsHSFA4a can bind to the OsUGT72F1 promoter and enhance its transcription level. Together, this study revealed that the glycosyltransferase gene OsUGT72F1 plays a vital role in the adaptive adjustment of high temperature stress in plants, revealing a new heat tolerance pathway and providing a promising gene candidate for the breeding of heat-resistant crop varieties.