SlNAC12, a novel NAC-type transcription factor, confers salt stress tolerance in tomato

SlNAC12 enhances salt stress tolerance of transgenic tomato by regulating ion homeostasis, antioxidant activity and flavonoids biosynthesis

Soil salinization is a major environmental factor that adversely affects plant growth and development. NAC (NAM, ATAF1/2, and CUC2) is a large family of plant-specific transcription factors that play crucial roles in stress response. Here, we investigated the role of a novel NAC transcription factor, SlNAC12, in conferring salt stress tolerance in tomato (Solanum lycopersicum). Subcellular localization and yeast assays studies revealed that SlNAC12 is localized in the nucleus with weak transcriptional activity. SlNAC12 transcript was induced by salt stress in the leaves and roots of tomato seedlings. Overexpression of SlNAC12 in tomato led to significantly reduced plant height and root length. Transgenic tomato lines overexpressing of SlNAC12 (OE#1 and OE#3) exhibited enhanced tolerance to salinity, as evidenced by reduced the inhibitory effect of growth parameters under salt stress compared to wild type (WT). Overexpression of SlNAC12 in tomato affected Na⁺ and K⁺ homeostasis, leading to reduced Na⁺/K⁺ ratio, enhanced activity of antioxidant enzymes and decreased reactive oxygen species (ROS) accumulation under salt stress. Furthermore, the transcript levels of several genes involved in flavonoids metabolism and the levels of flavonoids accumulation were increased in SlNAC12-overexpressing tomato lines. Collectively, this study suggests that SlNAC12 transcription factor enhances salt stress tolerance in tomato is correlated with ion homeostasis, antioxidant enzyme systems, and flavonoids accumulation.