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CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale

  • Dmitry Miroshnichenko,
  • Vadim Timerbaev,
  • Mikhail Divashuk,
  • Alexander Pushin,
  • Valeria Alekseeva,
  • Pavel Kroupin,
  • Mikhail Bazhenov,
  • Mariya Samarina,
  • Aleksey Ermolaev,
  • Gennady Karlov,
  • Sergey Dolgov

摘要

Key message

The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.