Scholarlink

Background <p>Conventional histopathology faces methodological limitations when assessing complex three-dimensional tissue architectures. In particular, for heterogeneous tissues such as the pancreas or in complex tissue pathologies, restriction to two-dimensional sections hampers comprehensive recognition of morphological features.</p> Objective <p>This study aims to demonstrate the potential of synchrotron-based phase-contrast imaging (SRµCT) as a&#xa0;tool for high-resolution visualization of pancreatic tissue. Three representative case examples were analyzed to capture morphological parameters volumetrically and correlate them with immunohistochemical marker profiles.</p> Materials and methods <p>Tissue cores from formalin-fixed, paraffin-embedded human pancreatic samples were volumetrically assessed using SRµCT. The investigated material was further processed as microarrays. Serial sections and immunohistochemical stains were correlated with the 3D datasets.</p> Results <p>SRµCT enabled detailed spatial visualization of functional compartments and neoplastic infiltration patterns. Non-neoplastic tissue revealed distinct morphological compartments. A&#xa0;well-differentiated neuroendocrine tumor exhibited trabecular architecture, whereas ductal adenocarcinoma displayed infiltrative growth with diffuse, heterogeneous architecture, irregular duct formations and stromal desmoplasia. Virtual slicing permitted orientation-independent analyses. Correlation with immunohistochemical profiles validated the morphofunctional findings.</p> Conclusion <p>SRµCT is a&#xa0;sensitive, non-invasive technique providing label-free 3D insights into pancreatic architecture. It opens new perspectives for research, teaching, and potentially advanced diagnostic applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Virtuelle Histopathologie des Pankreas: 3D-Einblicke mittels synchrotronbasierter Bildgebung

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月8日

查看全文
  • Matthias Martin Gaida,
  • Lukas Hessel,
  • Caroline Victoria Schimmel,
  • Klara Schulze,
  • Verena Wagner,
  • Philipp Mayer,
  • Jonas Albers,
  • Elizabeth Duke,
  • Martin Loos,
  • Gabriel Alexander Salg

摘要

Background

Conventional histopathology faces methodological limitations when assessing complex three-dimensional tissue architectures. In particular, for heterogeneous tissues such as the pancreas or in complex tissue pathologies, restriction to two-dimensional sections hampers comprehensive recognition of morphological features.

Objective

This study aims to demonstrate the potential of synchrotron-based phase-contrast imaging (SRµCT) as a tool for high-resolution visualization of pancreatic tissue. Three representative case examples were analyzed to capture morphological parameters volumetrically and correlate them with immunohistochemical marker profiles.

Materials and methods

Tissue cores from formalin-fixed, paraffin-embedded human pancreatic samples were volumetrically assessed using SRµCT. The investigated material was further processed as microarrays. Serial sections and immunohistochemical stains were correlated with the 3D datasets.

Results

SRµCT enabled detailed spatial visualization of functional compartments and neoplastic infiltration patterns. Non-neoplastic tissue revealed distinct morphological compartments. A well-differentiated neuroendocrine tumor exhibited trabecular architecture, whereas ductal adenocarcinoma displayed infiltrative growth with diffuse, heterogeneous architecture, irregular duct formations and stromal desmoplasia. Virtual slicing permitted orientation-independent analyses. Correlation with immunohistochemical profiles validated the morphofunctional findings.

Conclusion

SRµCT is a sensitive, non-invasive technique providing label-free 3D insights into pancreatic architecture. It opens new perspectives for research, teaching, and potentially advanced diagnostic applications.