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<p>Hyperbranched poly(2-(α-D-mannosoxy)ethyl methacrylate) (HBPManEMA) was synthesized via a one-pot method, i.e., the reversible addition-fragmentation chain transfer (RAFT) radical polymerization accompanied by the tandem reaction of Curtius rearrangement and CNO/OH coupling reaction with S-dodecyl-S’-(α, α’-dimethyl -α’’- acetyl azide)-trithiocarbonate as a functional chain transfer agent (CTA). The effects of the monomer-to-CTA ratio on the molecular parameters of HBPManEMA was investigated in detail. The results of multidetector Gel Permeation Chromatography confirmed that the degree of branching (DB) of HBPManEMA was controlled by the monomer-to-CTA ratio, and the dispersity (<i>Đ</i>) of HBPManEMA was kept at a low value. Moreover, a mechanism for this one-pot method was proposed with the assistance of elemental analysis, <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR), online GPC and Fourier transform infrared (FT-IR) spectroscopy. In addition, we further studied the complexation between HBPManEMA and concanavalin A (Con A), confirming that branched structure is conducive to the complexation, and the functionalized HNTs-KH570@HBPManEMA exhibiting superior separation efficiency. The developed system enables reversible lectin capture and competitive displacement, establishing a robust biomimetic platform for selective protein purification applications.</p>

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Engineering low-PDI hyperbranched polymannose via one-pot RAFT/tandem strategy for biomimetic lectin separation

  • 期刊论文

  • 发表日期：

    2026年7月7日

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  • Huijun Zhang,
  • Bo Zhao,
  • Liuyang Wang,
  • Liping Fang,
  • Feng Liu,
  • Hailei Zhang,
  • Yonggang Wu,
  • Xinwu Ba,
  • Libin Bai

摘要

Hyperbranched poly(2-(α-D-mannosoxy)ethyl methacrylate) (HBPManEMA) was synthesized via a one-pot method, i.e., the reversible addition-fragmentation chain transfer (RAFT) radical polymerization accompanied by the tandem reaction of Curtius rearrangement and CNO/OH coupling reaction with S-dodecyl-S’-(α, α’-dimethyl -α’’- acetyl azide)-trithiocarbonate as a functional chain transfer agent (CTA). The effects of the monomer-to-CTA ratio on the molecular parameters of HBPManEMA was investigated in detail. The results of multidetector Gel Permeation Chromatography confirmed that the degree of branching (DB) of HBPManEMA was controlled by the monomer-to-CTA ratio, and the dispersity (Đ) of HBPManEMA was kept at a low value. Moreover, a mechanism for this one-pot method was proposed with the assistance of elemental analysis, 1H nuclear magnetic resonance (1H NMR), online GPC and Fourier transform infrared (FT-IR) spectroscopy. In addition, we further studied the complexation between HBPManEMA and concanavalin A (Con A), confirming that branched structure is conducive to the complexation, and the functionalized HNTs-KH570@HBPManEMA exhibiting superior separation efficiency. The developed system enables reversible lectin capture and competitive displacement, establishing a robust biomimetic platform for selective protein purification applications.