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<p>Silk fibroin, a natural protein derived from silkworms, has gained significant attention in biomedical applications due to its excellent biocompatibility, biodegradability, and mechanical properties. This study focuses on the preparation and characterization of silk fibroin for wound healing applications. The fibroin was extracted through a controlled degumming process and characterized using SEM, FTIR, particle size, and zeta potential analyses to confirm its structural and morphological integrity. In vitro biocompatibility and cytotoxicity evaluations, including MTT assay, hemolysis, and coagulation studies, demonstrated its non-toxic nature and blood compatibility. Further, its wound healing potential was assessed through VEGF expression (angiogenesis), ROS assay (oxidative stress), and GSH assay (antioxidant response), revealing a significant improvement in cellular response. The results indicate that silk fibroin not only promotes cell viability but also enhances angiogenesis and antioxidant defense, making it a promising biomaterial for wound healing applications. The novelty of this study lies in the comprehensive evaluation of silk fibroin’s bioactivity, highlighting its role in oxidative stress modulation and vascularization, which are crucial factors for efficient wound repair. The findings contribute to the development of silk fibroin-based biomaterials for regenerative medicine, providing a sustainable and effective alternative for skin tissue engineering.</p>

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Physicochemical characterization and biocompatibility assessment of silk fibroin extracted from Bombyx mori

  • 期刊论文

  • 发表日期：

    2026年2月4日

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  • Subhasri Bogadi,
  • Md Ekhtiar Uddin,
  • Rokhsana Jahan,
  • Mohammed Abdul Kader,
  • Shahabe Saquib Abullais,
  • Md Shahin Reza

摘要

Silk fibroin, a natural protein derived from silkworms, has gained significant attention in biomedical applications due to its excellent biocompatibility, biodegradability, and mechanical properties. This study focuses on the preparation and characterization of silk fibroin for wound healing applications. The fibroin was extracted through a controlled degumming process and characterized using SEM, FTIR, particle size, and zeta potential analyses to confirm its structural and morphological integrity. In vitro biocompatibility and cytotoxicity evaluations, including MTT assay, hemolysis, and coagulation studies, demonstrated its non-toxic nature and blood compatibility. Further, its wound healing potential was assessed through VEGF expression (angiogenesis), ROS assay (oxidative stress), and GSH assay (antioxidant response), revealing a significant improvement in cellular response. The results indicate that silk fibroin not only promotes cell viability but also enhances angiogenesis and antioxidant defense, making it a promising biomaterial for wound healing applications. The novelty of this study lies in the comprehensive evaluation of silk fibroin’s bioactivity, highlighting its role in oxidative stress modulation and vascularization, which are crucial factors for efficient wound repair. The findings contribute to the development of silk fibroin-based biomaterials for regenerative medicine, providing a sustainable and effective alternative for skin tissue engineering.