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<p>Wound healing remains a significant challenge; moreover, in recent years, carbapenem-resistant <i>Acinetobacter baumannii</i> (<i>A. Baumannii</i>) classified as a critical-priority pathogen has been isolated from wounds, highlighting the need for alternative, natural treatment strategies. In this study, fig seed oil (FS) loaded chitosan hydrogels (CS) were prepared and characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). After confirming in vitro biocompatibility in L929 fibroblast cells, the antibacterial efficacy of these hydrogels against carbapenem-resistant <i>A. baumannii</i> was evaluated using disk diffusion and time-kill assays, and bacterial morphology following treatment was examined by SEM. Subsequently, in vivo wound healing experiments were performed. The synthesized hydrogels remained biocompatible even at the highest dose tested (1.5&#xa0;mg). FS-loaded CS hydrogels demonstrated strong antibacterial activity, with inhibition‐zone diameters increasing in a dose-dependent manner. SEM images revealed complete disruption of bacterial morphology after treatment. Furthermore, wound healing data indicated that FS was particularly effective: a wound measuring 4.81&#xa0;cm on day 0 was reduced to 1.24&#xa0;cm.</p>

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Fig seed oil–enriched Chitosan hydrogels: in vitro antibacterial activity against carbapenem-resistant Acinetobacter baumannii and in vivo wound healing evaluation

  • 期刊论文

  • 发表日期：

    2025年12月2日

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  • Metin Yildirim,
  • Kemal Dogan,
  • Akın Yiğin,
  • Mehmet Cimentepe,
  • Özge Öztürk Cimentepe,
  • Madina Amangeldinova

摘要

Wound healing remains a significant challenge; moreover, in recent years, carbapenem-resistant Acinetobacter baumannii (A. Baumannii) classified as a critical-priority pathogen has been isolated from wounds, highlighting the need for alternative, natural treatment strategies. In this study, fig seed oil (FS) loaded chitosan hydrogels (CS) were prepared and characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). After confirming in vitro biocompatibility in L929 fibroblast cells, the antibacterial efficacy of these hydrogels against carbapenem-resistant A. baumannii was evaluated using disk diffusion and time-kill assays, and bacterial morphology following treatment was examined by SEM. Subsequently, in vivo wound healing experiments were performed. The synthesized hydrogels remained biocompatible even at the highest dose tested (1.5 mg). FS-loaded CS hydrogels demonstrated strong antibacterial activity, with inhibition‐zone diameters increasing in a dose-dependent manner. SEM images revealed complete disruption of bacterial morphology after treatment. Furthermore, wound healing data indicated that FS was particularly effective: a wound measuring 4.81 cm on day 0 was reduced to 1.24 cm.