<p>The evaluation of ionic liquids (ILs), poly(ionic liquids) (PILs), and modified psyllium as lipid-binding agents was conducted using cholesterol as a model lipid. To explore the broader biomedical potential of these materials, the antifungal properties of the ILs were also assessed. Cholesterol uptake was investigated for P[VImBu]⁺Br⁻, P[VImBu]⁺CF₃COO⁻, P[VImBu]⁺SH⁻, and P[VImBu]⁺BF₄⁻, as well as for the parent polymer PVIm. Among these, P[VImBu]⁺Br⁻ exhibited the highest cholesterol uptake 71.60%, followed by IL-grafted psyllium (psy[VImS] + Br⁻) with 56.08%. Maximum percent binding (PB) was observed with mustard oil, with the binding trend decreasing in the order: mustard &gt; soybean &gt; sunflower oil. Similar binding patterns were observed across all systems, although IL-grafted psyllium showed anomalous PB behavior. Results indicated that the nature of both the cation and anion had a pronounced effect on antifungal performance. The ionic liquid [VImS]⁺SH⁻ shows the strongest antifungal efficacy against <i>Mucor circinelloides</i>, with the lowest MIC, outperforming other ILs and approaching the efficacy of the standard fungicide Thiophenate Methyl, highlighting the potential of ILs in antifungal applications. These findings open avenues for further in vivo and clinical studies to assess their safety and therapeutic potential in metabolic and infectious disease management.</p>