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<p>Chronic wound treatment serves as one of the major challenges faced by the healthcare sector. The utilization of nanomaterials for treating wounds serves as an efficient method for promoting wound healing and preventing microbial infections. In the current work, cerium oxide nanoparticles (CN) were prepared using <i>Plectranthus amboinicus</i> (Lour.) Spreng. leaf extract. The hydrodynamic diameter of CN was determined as 61.5&#xa0;nm with maximum UV absorption at 314&#xa0;nm. CN-incorporated polyacrylonitrile (CN-PAN) nanofibers were prepared using electrospinning method. The CN-PAN nanofibers appeared as continuous fibers disrupted with few beads when viewed under scanning electron microscope (SEM). In vitro blood clotting assay results revealed that CN-PAN nanofibers significantly lowered the time required for formation of blood clot when compared with control. Antibacterial activity of CN-PAN nanofibers was studied using spread plate method against <i>Escherichia coli</i>. MTT assay was carried out using HaCaT cells&#xa0;which showed that cell viability was not affected&#xa0;with the maximum concentration (30&#xa0;µg/ml) of CN-PAN nanofibers used for the experiment. In vitro scratch wound assay demonstrated that CN-PAN nanofibers can improve the migration of cells and result in faster closure of wounds. Chorioallantoic membrane (CAM) assay performed using fertilized chicken eggs showed new blood vessels formation in groups incubated with CN-PAN nanofibers. The enhanced hemostasis, antibacterial, cytocompatible, and wound healing properties of CN-PAN nanofibers make it a superior biomaterial in the field of medicine.</p> Graphical abstract <p></p>

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Fabrication of electrospun polymeric nanofibers incorporated with cerium oxide nanoparticles for enhanced hemostasis and wound healing

  • 期刊论文

  • 发表日期：

    2025年8月3日

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  • Sathyaraj Weslen Vedakumari,
  • Lokesh Prabakaran,
  • Priya Vadhana Palanisamy,
  • Yovan Raja Pravin,
  • Devanand Venkatasubbu

摘要

Chronic wound treatment serves as one of the major challenges faced by the healthcare sector. The utilization of nanomaterials for treating wounds serves as an efficient method for promoting wound healing and preventing microbial infections. In the current work, cerium oxide nanoparticles (CN) were prepared using Plectranthus amboinicus (Lour.) Spreng. leaf extract. The hydrodynamic diameter of CN was determined as 61.5 nm with maximum UV absorption at 314 nm. CN-incorporated polyacrylonitrile (CN-PAN) nanofibers were prepared using electrospinning method. The CN-PAN nanofibers appeared as continuous fibers disrupted with few beads when viewed under scanning electron microscope (SEM). In vitro blood clotting assay results revealed that CN-PAN nanofibers significantly lowered the time required for formation of blood clot when compared with control. Antibacterial activity of CN-PAN nanofibers was studied using spread plate method against Escherichia coli. MTT assay was carried out using HaCaT cells which showed that cell viability was not affected with the maximum concentration (30 µg/ml) of CN-PAN nanofibers used for the experiment. In vitro scratch wound assay demonstrated that CN-PAN nanofibers can improve the migration of cells and result in faster closure of wounds. Chorioallantoic membrane (CAM) assay performed using fertilized chicken eggs showed new blood vessels formation in groups incubated with CN-PAN nanofibers. The enhanced hemostasis, antibacterial, cytocompatible, and wound healing properties of CN-PAN nanofibers make it a superior biomaterial in the field of medicine.

Graphical abstract