<p>The present work aims at the preparation of a novel cost-effective sensor material for the detection of cyanamide. The best part of the present work is that it is the first time to introduce a technique for cyanamide sensing. No other literature works are yet reported for the electrochemical sensing of cyanamide molecule; it is confirmed from the literature reports. It consists of preparation of graphene oxide (GO) from graphite powder by using modified Hummer’s method. Functionalized GO-based molecularly imprinted polymer (MIP) is used for the detection of cyanamide. First GO functionalization is done with para-aminophenol (PAP) by solvent sonication giving GO-PAP; then, it undergoes surface modification with allyl bromide in the presence of sodium hydroxide (NaOH) and dimethyl sulfoxide (DMSO) to form vinylated GO (GO–O–CH₂–CH=CH₂). The modified GO undergoes polymerization with methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, azobisisobutyronitrile (AIBN) as the initiator and cyanamide as the template molecule. The cyanamide is completely removed by using N, N dimethylformamide (DMF), and a non-imprinted polymer (NIP) is also prepared along with MIP. Different materials obtained in each stage of preparation were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscope (SEM). The prepared GO-MIP is used as an active material for cyanamide sensing where the sensor exhibits a lower limit detection of 0.1&#xa0;nM with maximum sensing performance at pH 8.0 and a long-term stability for more than six months. The interference of cyanamide with thiourea (TU), 2,4-dichlorophenoxyacetic acid (DPA) and 2,4,5-trichlorophenoxyacetic acid (TPA) was also studied. The GO-MIP shows a negligible DPA, TPA, modest TU interferences and better performance in cyanamide sensing, proposing the real-time usability of the sensor.</p>