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<p>A promising biocomposite was synthesized via ultrasonication using cerium-substituted hydroxyapatite (Ce-HAP) derived from crab shells, sodium alginate (SA) and <i>Wedelia chinensis</i> leaf extract (WLE) for bone healing applications. Cerium (Ce) ions substituted in the HAP improves osseointegration and antibacterial activity, probably aiding in bone repair. Also, the SA was employed as a compositing agent due to its biodegradable, non-toxic and bioactivity in nature. In addition, WLE is well recognized for its antimicrobial, antioxidant, anticancer, anti-inflammatory, wound healing properties, etc. The functional groups, phase, structure, elemental composition and thermal stability of the as-synthesized Ce-HAP/SA/WLE biocomposite were characterized by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray analysis (EDAX-mapping), Transmission electron microscopy (TEM), and Thermogravimetric analysis (TGA). The mechanical strength of this biocomposite was evaluated by the Vickers microhardness test. Further, antibacterial properties of the biocomposite were assessed against <i>Staphylococcus aureus (S. aureus)</i> and <i>Escherichia coli (E. coli)</i>. In vitro bioactivity was examined by immersing HAP, Ce-HAP, Ce-HAP/SA and Ce-HAP/SA/WLE biocomposite in simulated body fluid (SBF) for 14&#xa0;days. The osteosarcoma cancer (MG63) cells were treated with biocomposite at various concentrations of 6.5, 12.5, 25, 50 and 100&#xa0;μg/mL for 48&#xa0;h of incubation. Hence, the as-synthesized Ce-HAP/SA/WLE biocomposite exhibited improved thermal, mechanical and biological activities, indicating a significant potential for bone healing applications.</p> Graphical abstract <p></p>

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Investigation on the physicochemical and biological properties of crab shells-derived Ce-HAP/sodium alginate/Wedelia chinensis plant extract-incorporated biocomposite for bone healing applications

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Merina Kaveri,
  • Kavitha Louis,
  • Gopi Dhanaraj

摘要

A promising biocomposite was synthesized via ultrasonication using cerium-substituted hydroxyapatite (Ce-HAP) derived from crab shells, sodium alginate (SA) and Wedelia chinensis leaf extract (WLE) for bone healing applications. Cerium (Ce) ions substituted in the HAP improves osseointegration and antibacterial activity, probably aiding in bone repair. Also, the SA was employed as a compositing agent due to its biodegradable, non-toxic and bioactivity in nature. In addition, WLE is well recognized for its antimicrobial, antioxidant, anticancer, anti-inflammatory, wound healing properties, etc. The functional groups, phase, structure, elemental composition and thermal stability of the as-synthesized Ce-HAP/SA/WLE biocomposite were characterized by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray analysis (EDAX-mapping), Transmission electron microscopy (TEM), and Thermogravimetric analysis (TGA). The mechanical strength of this biocomposite was evaluated by the Vickers microhardness test. Further, antibacterial properties of the biocomposite were assessed against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). In vitro bioactivity was examined by immersing HAP, Ce-HAP, Ce-HAP/SA and Ce-HAP/SA/WLE biocomposite in simulated body fluid (SBF) for 14 days. The osteosarcoma cancer (MG63) cells were treated with biocomposite at various concentrations of 6.5, 12.5, 25, 50 and 100 μg/mL for 48 h of incubation. Hence, the as-synthesized Ce-HAP/SA/WLE biocomposite exhibited improved thermal, mechanical and biological activities, indicating a significant potential for bone healing applications.

Graphical abstract