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<p>Hydrogels are highly preferred for wound dressing applications due to their biocompatibility, biodegradability, high water retention capacity, and similarity to extracellular matrix (ECM). In the present study, a novel hydrogel for wound management has been developed by incorporating <i>Couroupita guianensis</i> flower extract (CGFE) into a polymer composite of polyvinyl alcohol (PVA), chitosan (CS), and collagen hydrolysate (CA). The metabolite profile of CGFE, as identified through the GC–MS/MS, has demonstrated its potential for wound healing. Here, two bioactive compounds, such as 2-amino-5-methylbenzoic acid and 2-hydroxy-3-O-tigloyl-6-O-acetylswietenolide, with antimicrobial and antioxidant properties, have&#xa0;been identified from the CGFE. The hydrogels were subjected to characterisation by UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FE-SEM), along with its functional validation through cytotoxicity and scratch wound assays. The hydrodynamic properties of the hydrogels have also been observed to be supportive for its potential for wound-healing applications. By the FE-SEM analysis, the prepared hydrogel was found to have a&#xa0;highly porous structure, which could facilitate the fluid and gas exchange. The developed hydrogel exhibited a significant inhibitory effect (20.6 ± 0.94&#xa0;mm) against the multidrug-resistant <i>Staphylococcus aureus</i>. Further, cytotoxicity assay demonstrated 92.14% cell viability at the hydrogel concentration of 12.5&#xa0;µg/mL. At the same time, the prepared hydrogel resulted in the wound closure within 48&#xa0;h, as experimentally validated by scratch assay. These findings highlight the potential of the PVA–chitosan–collagen hydrogel system, incorporated with <i>C. guianensis</i> flower extract, to be a promising candidate for advanced wound healing with notable activity against drug-resistant wound pathogens.</p>

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Biocompatible PVA–chitosan hydrogel enriched with collagen hydrolysate and Couroupita guianensis flower extract for enhanced wound-healing and antibacterial activity

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Aparna G. Kumar,
  • Arun Nair,
  • S. Sreejith,
  • D. Adithya,
  • Jiya Jose,
  • Vipina Vinod Thazhenandayipurath,
  • K. Sreekanth,
  • C. Chandana,
  • N. Radhakrishnan,
  • E. K. Radhakrishnan

摘要

Hydrogels are highly preferred for wound dressing applications due to their biocompatibility, biodegradability, high water retention capacity, and similarity to extracellular matrix (ECM). In the present study, a novel hydrogel for wound management has been developed by incorporating Couroupita guianensis flower extract (CGFE) into a polymer composite of polyvinyl alcohol (PVA), chitosan (CS), and collagen hydrolysate (CA). The metabolite profile of CGFE, as identified through the GC–MS/MS, has demonstrated its potential for wound healing. Here, two bioactive compounds, such as 2-amino-5-methylbenzoic acid and 2-hydroxy-3-O-tigloyl-6-O-acetylswietenolide, with antimicrobial and antioxidant properties, have been identified from the CGFE. The hydrogels were subjected to characterisation by UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FE-SEM), along with its functional validation through cytotoxicity and scratch wound assays. The hydrodynamic properties of the hydrogels have also been observed to be supportive for its potential for wound-healing applications. By the FE-SEM analysis, the prepared hydrogel was found to have a highly porous structure, which could facilitate the fluid and gas exchange. The developed hydrogel exhibited a significant inhibitory effect (20.6 ± 0.94 mm) against the multidrug-resistant Staphylococcus aureus. Further, cytotoxicity assay demonstrated 92.14% cell viability at the hydrogel concentration of 12.5 µg/mL. At the same time, the prepared hydrogel resulted in the wound closure within 48 h, as experimentally validated by scratch assay. These findings highlight the potential of the PVA–chitosan–collagen hydrogel system, incorporated with C. guianensis flower extract, to be a promising candidate for advanced wound healing with notable activity against drug-resistant wound pathogens.