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<p>Lipopeptides, known for their amphipathic nature and surface activity, hold great potential as sustainable and non-toxic products. However, their commercial availability remains limited due to high production costs. This study presents a low-cost culture medium formulated with soybean meal extract and purifies a lipopeptide from <i>Bacillus subtilis</i> ATCC 19659. The lipopeptide was thoroughly characterized biochemically and in silico. Physiological experiments determined key parameters such as critical micelle concentration, surface tension, stability, oil dispersion, and toxicity. Our findings reveal that the yield of purified lipopeptide was 1.43&#xa0;g&#xa0;L<sup>−1</sup> and was classified as iturinaA, featuring a primary structure comprising <span>l</span>-Asn, <span>d</span>-Tyr, <span>d</span>-Asn, <span>l</span>-Gln, <span>l</span>-Pro, <span>d</span>-Asn, and <span>l</span>-Ser in two isoforms (C13 and C14). IturinA exhibited remarkable properties, including a critical micelle concentration of 0.083&#xa0;mg&#xa0;mL<sup>−1</sup>, the Interfacial tension at 10&#xa0;mg&#xa0;mL<sup>−1</sup> was 1&#xa0;mN&#xa0;m<sup>−1</sup> (± 0.1); an average contact angle of 54.74° (± 1.6), EI<sub>24</sub> for long chain hydrocarbons was 85%, stability under pH, salt, and temperature, excellent oil dispersion activity, and non-toxicity towards <i>Artemia salina</i>. These results highlight the promising future of IturinA in various biotechnological applications.</p> Graphical abstract <p></p>

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Eco-friendly IturinA lipopeptide: biochemical characterization for unlocking potential for enhanced oil recovery

  • 期刊论文

  • 发表日期：

    2025年5月31日

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  • Diego Fernandes Livio,
  • Danielle de Almeida,
  • Hiure Gomes Ramos Meira,
  • Maria Auxiliadora de Oliveira,
  • Luisa Ferreira da Cruz,
  • Vinícius Souza Tarabal,
  • Bruno Marques Silva,
  • Júlia Teixeira Rodrigues,
  • Pedro Gontijo Carneiro,
  • Adriano Guimarães Parreira,
  • José Antonio da Silva,
  • Marina Quádrio Raposo Branco Rodrigues,
  • Ronaldo Adriano Ribeiro,
  • Daniel Bonoto Gonçalves,
  • Mariana Torquato Quezado de Magalhães,
  • Paulo Afonso Granjeiro

摘要

Lipopeptides, known for their amphipathic nature and surface activity, hold great potential as sustainable and non-toxic products. However, their commercial availability remains limited due to high production costs. This study presents a low-cost culture medium formulated with soybean meal extract and purifies a lipopeptide from Bacillus subtilis ATCC 19659. The lipopeptide was thoroughly characterized biochemically and in silico. Physiological experiments determined key parameters such as critical micelle concentration, surface tension, stability, oil dispersion, and toxicity. Our findings reveal that the yield of purified lipopeptide was 1.43 g L−1 and was classified as iturinaA, featuring a primary structure comprising l-Asn, d-Tyr, d-Asn, l-Gln, l-Pro, d-Asn, and l-Ser in two isoforms (C13 and C14). IturinA exhibited remarkable properties, including a critical micelle concentration of 0.083 mg mL−1, the Interfacial tension at 10 mg mL−1 was 1 mN m−1 (± 0.1); an average contact angle of 54.74° (± 1.6), EI24 for long chain hydrocarbons was 85%, stability under pH, salt, and temperature, excellent oil dispersion activity, and non-toxicity towards Artemia salina. These results highlight the promising future of IturinA in various biotechnological applications.

Graphical abstract