Scholarlink

<p>Antifungal drug resistance in <i>Trichophyton interdigitale</i> reinforces the need for new drugs with multiple targets. The antifungal activity of <i>Eruca sativa</i> seed oil, clove oil, and ethanolic extract of <i>Fusarium chlamydosporum</i> was evaluated against a clinically isolated <i>T. interdigitale</i> Accession No. ON564615 that was later molecularly confirmed. The Gas Chromatography Mass Spectrometry GC MS method was used to profile the bioactive constituents. In order to analyze their high affinity binding to several important enzymes of the ergosterol pathway such as squalene epoxidase ERG1, C 24 sterol methyltransferase ERG6, and lanosterol 14α demethylase ERG11, their multi target effects were systematically analyzed using integrated molecular docking simulations. In this study <i>E. sativa</i> oil exhibited significant activity yielding an inhibition zone of 57.7 mm and an MIC of 6.25 mg/mL. The effect of <i>F. chlamydosporum</i> extract in combination with clove oil was remarkably strong showing 72.67 mm of inhibition which is higher than that of the first line drug terbinafine 61 mm. In addition the presence of eugenol in clove oil and of ethyl iso allocholate in <i>E. sativa</i> and the fungal extracts was detected by GC MS analysis. The present data are limited to in vitro and in silico testing of a single clinical isolate; however the findings are highly suggestive of the potential of using <i>E. sativa</i> oil and or certain specific combinations of fungal and plant extracts as promising multi target antifungals. In vivo studies are required in the future to fully confirm their clinical translatability against resistant dermatophytosis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In Vitro Antifungal Evaluation, GC–MS Profiling, and Molecular Docking of Eruca sativa Oil, Clove Oil, and Fusarium chlamydosporum Extract Against the Dermatophyte Trichophyton interdigitale

  • 期刊论文

  • 发表日期：

    2026年7月16日

查看全文
  • Yasmin M. Elsaba,
  • Mohamed E. Osman,
  • Ayman F. Ahmed,
  • Mona Mohamed Darwish Hegazy,
  • Hager Hussein Abdel karim,
  • Eslam T. Mohamed

摘要

Antifungal drug resistance in Trichophyton interdigitale reinforces the need for new drugs with multiple targets. The antifungal activity of Eruca sativa seed oil, clove oil, and ethanolic extract of Fusarium chlamydosporum was evaluated against a clinically isolated T. interdigitale Accession No. ON564615 that was later molecularly confirmed. The Gas Chromatography Mass Spectrometry GC MS method was used to profile the bioactive constituents. In order to analyze their high affinity binding to several important enzymes of the ergosterol pathway such as squalene epoxidase ERG1, C 24 sterol methyltransferase ERG6, and lanosterol 14α demethylase ERG11, their multi target effects were systematically analyzed using integrated molecular docking simulations. In this study E. sativa oil exhibited significant activity yielding an inhibition zone of 57.7 mm and an MIC of 6.25 mg/mL. The effect of F. chlamydosporum extract in combination with clove oil was remarkably strong showing 72.67 mm of inhibition which is higher than that of the first line drug terbinafine 61 mm. In addition the presence of eugenol in clove oil and of ethyl iso allocholate in E. sativa and the fungal extracts was detected by GC MS analysis. The present data are limited to in vitro and in silico testing of a single clinical isolate; however the findings are highly suggestive of the potential of using E. sativa oil and or certain specific combinations of fungal and plant extracts as promising multi target antifungals. In vivo studies are required in the future to fully confirm their clinical translatability against resistant dermatophytosis.