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<p>A Gram-stain-positive bacterium capable of producing siderophore and indole-3-acetic acid (IAA), designated strain H7<sup>T</sup>, was isolated from soil of grassland in Nanchang, Jiangxi Province. Cells were rod-shaped, facultatively anaerobic, endospore-forming and motile by peritrichous flagella. Cells grow at 15–42&#xa0;°C (optimum, 30&#xa0;°C), pH 5–9 (optimum, pH 7) and with 0–10% NaCl (w/v; optimum, 2%). 16S rRNA gene sequence analysis showed that strain H7<sup>T</sup> belonged to the genus <i>Brevibacillus</i> and was most related to <i>B. brevis</i> NBRC 15304<sup>T</sup> (96.7%), <i>B. fortis</i> NRRL NRS-1210<sup>T</sup> (96.7%), <i>B. formosus</i> DSM 9885<sup>T</sup> (96.6%), and <i>B. porteri</i> NRRL B-41110<sup>T</sup> (96.6%). Phylogenetic tree based on genome and 16S rRNA gene sequences indicated that strain H7<sup>T</sup> was clustered in the genus <i>Brevibacillus</i> but formed a separate clade. The genome size of H7<sup>T</sup> was 4.05&#xa0;Mb. The nucleotide identity (ANI) value and digital DNA–DNA hybridization (dDDH) between the genomes of strain H7<sup>T</sup> and the closely related type strains ranged from 71.2 to 73.1% and 14.9–20.5%, respectively. The major cellular fatty acids (≥ 5%) are anteiso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, C<sub>16:1</sub><i>ω</i>7calcohol, iso-C<sub>14:0</sub> and iso-C<sub>15:0</sub>. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME) and one unidentified lipid (UL). The predominant respiratory quinone was MK-7. The DNA G + C content was 52.1%. Based on phylogenetic, genomic, chemotaxonomic and phenotypic data, strain H7<sup>T</sup> (<b>=</b> MCCC 1K09426<sup>T</sup>= KCTC 43803<sup>T</sup>) was considered to represent a novel species of genus <i>Brevibacillus</i> which the name <i>Brevibacillus soli</i> sp. nov. is proposed.</p>

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Brevibacillus soli sp. nov., a Siderophore and IAA-Producing Strain Isolated from Soil

  • 期刊论文

  • 发表日期：

    2026年4月17日

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  • Jiale Ma,
  • Shijie Xiang,
  • Sihang Liu,
  • Minggen Cheng,
  • Jiguo Qiu,
  • Jian He,
  • Zongzhuan Shen,
  • Jiandong Jiang,
  • Qirong Shen

摘要

A Gram-stain-positive bacterium capable of producing siderophore and indole-3-acetic acid (IAA), designated strain H7T, was isolated from soil of grassland in Nanchang, Jiangxi Province. Cells were rod-shaped, facultatively anaerobic, endospore-forming and motile by peritrichous flagella. Cells grow at 15–42 °C (optimum, 30 °C), pH 5–9 (optimum, pH 7) and with 0–10% NaCl (w/v; optimum, 2%). 16S rRNA gene sequence analysis showed that strain H7T belonged to the genus Brevibacillus and was most related to B. brevis NBRC 15304T (96.7%), B. fortis NRRL NRS-1210T (96.7%), B. formosus DSM 9885T (96.6%), and B. porteri NRRL B-41110T (96.6%). Phylogenetic tree based on genome and 16S rRNA gene sequences indicated that strain H7T was clustered in the genus Brevibacillus but formed a separate clade. The genome size of H7T was 4.05 Mb. The nucleotide identity (ANI) value and digital DNA–DNA hybridization (dDDH) between the genomes of strain H7T and the closely related type strains ranged from 71.2 to 73.1% and 14.9–20.5%, respectively. The major cellular fatty acids (≥ 5%) are anteiso-C15:0, iso-C16:0, C16:1ω7calcohol, iso-C14:0 and iso-C15:0. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME) and one unidentified lipid (UL). The predominant respiratory quinone was MK-7. The DNA G + C content was 52.1%. Based on phylogenetic, genomic, chemotaxonomic and phenotypic data, strain H7T (= MCCC 1K09426T= KCTC 43803T) was considered to represent a novel species of genus Brevibacillus which the name Brevibacillus soli sp. nov. is proposed.