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<p>A Gram-stain-positive, aerobic, rod-shaped and non-motile bacterium, designated strain JX24<sup>T</sup>, was isolated from cornfield soil in Jiangxi Province, China. Growth was observed at 20–50&#xa0;°C (optimum 37&#xa0;°C), pH 6.0–10.0 (optimum pH 8.0) and salinity of 0–8.0% (w/v; optimum 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JX24<sup>T</sup> belongs to the genus <i>Metabacillus</i> and exhibits the highest sequence similarity to <i>Metabacillus indicus</i> LMG 22858<sup>T</sup> (99.9%) and <i>Metabacillus idriensis</i> SMC 4352-2<sup>T</sup> (98.2%). The draft genome of strain JX24<sup>T</sup> was approximately 4.2&#xa0;Mb with a 44.3% G + C content. The average nucleotide identity values between JX24<sup>T</sup> and the most closely related type strains <i>M. indicus</i> LMG 22858<sup>T</sup> and <i>M. idriensis</i> SMC 4352-2<sup>T</sup> were 93.1% and 77.2%, respectively, and the digital DNA–DNA hybridization values between them were 50.8% and 21.1%, respectively. The predominant fatty acids (≥ 5% of the total) of strain JX24<sup>T</sup> were <i>iso</i>-C<sub>15:0</sub>, <i>anteiso</i>-C<sub>15:0</sub>, <i>iso</i>-C<sub>16:0</sub>, <i>iso</i>-C<sub>17:0</sub> and <i>anteiso</i>-C<sub>17:0</sub>. The major isoprenoid quinone was MK-7. The major polar lipids of strain JX24<sup>T</sup> were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids, phospholipid and aminophosphoglycolipid. Based on phenotypic, genotypic and phylogenetic evidence, strain JX24<sup>T</sup> represents a novel species in the genus <i>Metabacillus</i>, for which the name <i>Metabacillus mesotrionivorans</i> sp. nov. is proposed. The type strain is JX24<sup>T</sup> (= GDMCC 1.4914<sup>T</sup> = KCTC 43837<sup>T</sup> = KACC 24116<sup>T</sup>). Furthermore, a NADPH-dependent nitroreductase MnrC, which efficiently transformed mesotrione to AMBA was identified in strain JX24<sup>T</sup>.</p>

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Metabacillus mesotrionivorans sp. nov., a Mesotrione‑Degrading Bacterium Isolated from Cornfield Soil

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Jialu Li,
  • Huipeng Wu,
  • Miaohuan Xiong,
  • Jian He,
  • Zhiming Wu,
  • Bin Liu,
  • Haiyan Ni

摘要

A Gram-stain-positive, aerobic, rod-shaped and non-motile bacterium, designated strain JX24T, was isolated from cornfield soil in Jiangxi Province, China. Growth was observed at 20–50 °C (optimum 37 °C), pH 6.0–10.0 (optimum pH 8.0) and salinity of 0–8.0% (w/v; optimum 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JX24T belongs to the genus Metabacillus and exhibits the highest sequence similarity to Metabacillus indicus LMG 22858T (99.9%) and Metabacillus idriensis SMC 4352-2T (98.2%). The draft genome of strain JX24T was approximately 4.2 Mb with a 44.3% G + C content. The average nucleotide identity values between JX24T and the most closely related type strains M. indicus LMG 22858T and M. idriensis SMC 4352-2T were 93.1% and 77.2%, respectively, and the digital DNA–DNA hybridization values between them were 50.8% and 21.1%, respectively. The predominant fatty acids (≥ 5% of the total) of strain JX24T were iso-C15:0, anteiso-C15:0, iso-C16:0, iso-C17:0 and anteiso-C17:0. The major isoprenoid quinone was MK-7. The major polar lipids of strain JX24T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids, phospholipid and aminophosphoglycolipid. Based on phenotypic, genotypic and phylogenetic evidence, strain JX24T represents a novel species in the genus Metabacillus, for which the name Metabacillus mesotrionivorans sp. nov. is proposed. The type strain is JX24T (= GDMCC 1.4914T = KCTC 43837T = KACC 24116T). Furthermore, a NADPH-dependent nitroreductase MnrC, which efficiently transformed mesotrione to AMBA was identified in strain JX24T.