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<p>Bioremediation has emerged as a crucial strategy for addressing oil pollution, with proteomics providing direct insights into microbial response to hydrocarbon exposure. However, the molecular mechanisms underlying hydrocarbon response and degradation in <i>Acinetobacter</i> species remain poorly understood. In this study, quantitative proteomic analysis using iTRAQ technology was performed on <i>Acinetobacter</i> sp. HC8-3&#xa0;S following exposure to hydrocarbons for 5 days. A total 87 differentially expressed proteins were identified, with 72 upregulated and 15 downregulated. These proteins were classified into 12 functional categories based on KEGG pathways and relevant literature, with a significant portion involved in the fatty acid degradation pathway. Notably, alkane degradation and fatty acid oxidation genes were highlighted as key contributors to hydrocarbons degradation. These findings confirm the ability of strain HC8-3&#xa0;S to degrade n-alkanes, underscoring its potential application in the bioremediation of crude oil-contaminated environments. The integrative analysis of proteomic data and functional genes provides valuable insights into the adaptive mechanism of <i>Acinetobacter</i> sp. HC8-3&#xa0;S and offers promising directions for developing sustainable solutions for hydrocarbon pollution.</p>

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Quantitative Proteomic Analysis of Differential Proteins from Acinetobacter sp. HC8-3S Responses to Hydrocarbons

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Yachao Kong,
  • Jun Min,
  • Yuhua Liu,
  • Suyun Fang,
  • Yanyan Huang,
  • Yanhong Xu,
  • Xiaoke Hu

摘要

Bioremediation has emerged as a crucial strategy for addressing oil pollution, with proteomics providing direct insights into microbial response to hydrocarbon exposure. However, the molecular mechanisms underlying hydrocarbon response and degradation in Acinetobacter species remain poorly understood. In this study, quantitative proteomic analysis using iTRAQ technology was performed on Acinetobacter sp. HC8-3 S following exposure to hydrocarbons for 5 days. A total 87 differentially expressed proteins were identified, with 72 upregulated and 15 downregulated. These proteins were classified into 12 functional categories based on KEGG pathways and relevant literature, with a significant portion involved in the fatty acid degradation pathway. Notably, alkane degradation and fatty acid oxidation genes were highlighted as key contributors to hydrocarbons degradation. These findings confirm the ability of strain HC8-3 S to degrade n-alkanes, underscoring its potential application in the bioremediation of crude oil-contaminated environments. The integrative analysis of proteomic data and functional genes provides valuable insights into the adaptive mechanism of Acinetobacter sp. HC8-3 S and offers promising directions for developing sustainable solutions for hydrocarbon pollution.