Scholarlink

<p>This study investigated the potential of <i>Paracoccus shandongensis</i> wg2, a strain isolated from propylene oxide (PO) saponification wastewater-activated sludge, for synthesizing polyhydroxybutyrate-co-hydroxyvalerate (PHBV) using sodium acetate as the sole carbon source. The synthesis of PHBV was confirmed by nuclear magnetic resonance (NMR) analysis, eliminating the need for 3-hydroxyvalerate (3-HV) precursors and highlighting the strain’s potential for significantly reducing production costs. Initial optimization using a single-factor approach was followed by a response surface methodology employing Plackett-Burman and Box-Behnken experimental designs. The optimal conditions were determined as follows: sodium acetate concentration of 7.5&#xa0;g/L, fermentation temperature of 29&#xa0;°C, and loading volume of 120 mL, demonstrating the higher PHBV yield of strain wg2 (41.19%), representing an 11.87% enhancement compared to the unoptimized conditions. The wg2 based synthesized PHBV demonstrated high thermal stability and remarkable physical and mechanical properties, indicating significant potential in thermoplastic applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of PHBV Synthesis Using Acetate as a Carbon Source by Paracoccus shandongensis wg2 via Response Surface Methodology

  • 期刊论文

  • 发表日期：

    2026年3月24日

查看全文
  • Yu Gan,
  • Fanzhe Chang,
  • Chengjiao Yu,
  • Huijie Liu,
  • Xiujun Zhang,
  • Qiang Li

摘要

This study investigated the potential of Paracoccus shandongensis wg2, a strain isolated from propylene oxide (PO) saponification wastewater-activated sludge, for synthesizing polyhydroxybutyrate-co-hydroxyvalerate (PHBV) using sodium acetate as the sole carbon source. The synthesis of PHBV was confirmed by nuclear magnetic resonance (NMR) analysis, eliminating the need for 3-hydroxyvalerate (3-HV) precursors and highlighting the strain’s potential for significantly reducing production costs. Initial optimization using a single-factor approach was followed by a response surface methodology employing Plackett-Burman and Box-Behnken experimental designs. The optimal conditions were determined as follows: sodium acetate concentration of 7.5 g/L, fermentation temperature of 29 °C, and loading volume of 120 mL, demonstrating the higher PHBV yield of strain wg2 (41.19%), representing an 11.87% enhancement compared to the unoptimized conditions. The wg2 based synthesized PHBV demonstrated high thermal stability and remarkable physical and mechanical properties, indicating significant potential in thermoplastic applications.