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<p>The present study evaluates the antibacterial efficacy of a bacteriocin synthesized by <i>Lactobacillus helveticus</i> PMP-6 (GenBank accession no. MT444488) against <i>Xanthomonas axonopodis pv. punicae</i> (Xpp-1), the etiological agent of bacterial blight in <i>Punica granatum L.</i> (Pomegranate). The bacteriocin underwent purification, leading to a substantial increase in its specific activity from 233.849 AUmg-1 to 3615.81 AUmg-1, confirming successful enrichment of the bioactive peptide. Antibacterial assays demonstrated inhibitory effects under optimal conditions of 40&#xa0;°C and pH 7.0, with a Minimum Inhibitory Concentration of 300 µgmL-1 and a Minimum Bactericidal Concentration of 400 µgmL-1. The antibacterial effect diminished in the presence of proteolytic enzymes, suggesting its proteinaceous nature. SDS-PAGE profiling of purified bacteriocin revealed a molecular weight of approximately 17&#xa0;kDa. Furthermore, a Detached Leaf Assay validated it’s in planta effectiveness, as no visible disease symptoms were observed in treated pomegranate leaves up to 96&#xa0;h. of post-inoculation. Collectively, these results underscore the potential of <i>L. helveticus</i> PMP-6-derived bacteriocin as a environmentally sustainable biocontrol agent for managing bacterial blight disease of pomegranate, providing a viable alternative to conventional chemical pesticides.</p> Graphical Abstract <p></p>

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Eco-friendly Suppression of Xanthomonas axonopodis pv. punicae: the Role of Lactobacillus helveticus PMP-6 in Pomegranate Disease Management

  • 期刊论文

  • 发表日期：

    2026年2月18日

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  • Pravin Vasantrao Deshmukh,
  • Prakash Ramrao Thorat,
  • Sopan Ganpatrao Wagh,
  • Akshay Milind Patil,
  • Vrunal Vishwasrao More,
  • Rohini Ravindra Shivshran

摘要

The present study evaluates the antibacterial efficacy of a bacteriocin synthesized by Lactobacillus helveticus PMP-6 (GenBank accession no. MT444488) against Xanthomonas axonopodis pv. punicae (Xpp-1), the etiological agent of bacterial blight in Punica granatum L. (Pomegranate). The bacteriocin underwent purification, leading to a substantial increase in its specific activity from 233.849 AUmg-1 to 3615.81 AUmg-1, confirming successful enrichment of the bioactive peptide. Antibacterial assays demonstrated inhibitory effects under optimal conditions of 40 °C and pH 7.0, with a Minimum Inhibitory Concentration of 300 µgmL-1 and a Minimum Bactericidal Concentration of 400 µgmL-1. The antibacterial effect diminished in the presence of proteolytic enzymes, suggesting its proteinaceous nature. SDS-PAGE profiling of purified bacteriocin revealed a molecular weight of approximately 17 kDa. Furthermore, a Detached Leaf Assay validated it’s in planta effectiveness, as no visible disease symptoms were observed in treated pomegranate leaves up to 96 h. of post-inoculation. Collectively, these results underscore the potential of L. helveticus PMP-6-derived bacteriocin as a environmentally sustainable biocontrol agent for managing bacterial blight disease of pomegranate, providing a viable alternative to conventional chemical pesticides.

Graphical Abstract